Breakthrough in P450 bioelectrochemistry and future perspectives

Improving the electrochemical performance of cytochrome P450 enzymes is highly desirable due to their versatility in the recognition of different biological and xenobiotic compounds. The task poses an exciting challenge because it leads not only to the acquisition of fundamental knowledge on the red...

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Bibliographic Details
Published in:Biochimica et biophysica acta 2011, Vol.1814 (1), p.237-248
Main Authors: Sadeghi, Sheila J., Fantuzzi, Andrea, Gilardi, Gianfranco
Format: Article
Language:English
Subjects:
Biocatalysis
Biotechnology
Cytochrome P-450 Enzyme System - chemistry
Cytochrome P-450 Enzyme System - metabolism
Cytochrome P450
Electrochemical Techniques - instrumentation
Electrochemical Techniques - methods
Electrochemical Techniques - trends
Electrochemistry
Electrodes
Electron transfer
Enzymes, Immobilized - chemistry
Enzymes, Immobilized - metabolism
Forecasting
Immobilisation
Models, Molecular
Oxidation-Reduction
Protein Conformation
Protein Structure, Secondary
Substrate turnover
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Summary:Improving the electrochemical performance of cytochrome P450 enzymes is highly desirable due to their versatility in the recognition of different biological and xenobiotic compounds. The task poses an exciting challenge because it leads not only to the acquisition of fundamental knowledge on the redox properties of these enzymes, but it also opens opportunities for technological and commercial applications. Interfacing these enzymes to electrode surfaces and electrochemically driving their catalytic cycle has proven to be very difficult. Initial attempts made by several groups included the direct immobilisation of these enzymes on electrode surfaces and omission of their redox partners for simplification of their electron transfer pathway. The data obtained in these cases generally resulted in a high heterogeneous electron transfer rate but without success in terms of detectable substrate turnover. The breakthrough in electrocatalysis has been made when both the electrode and the enzyme have been engineered, in some cases mimicking the natural environment of the microsomal enzymes and the inclusion of their electron transfer partners. This paper reviews and discusses the recent literature on this subject, and highlights the different approaches that have led to an unprecedented advancement of this area of research.
ISSN:1570-9639
0006-3002
1878-1454
DOI:10.1016/j.bbapap.2010.07.010