Proteome analysis of aniline-induced proteins in Acinetobacter lwoffii K24

Acinetobacter lwoffii K24 is a soil bacterium that can use aniline as a sole carbon and nitrogen source (by beta-ketoadipate pathway genes (cat genes)) and has two copies of catABC gene separately located on the chromosome. In order to identify aniline-induced proteins, two-dimensional electrophores...

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Bibliographic Details
Published in:Current microbiology 2002, Vol.44 (1), p.61-66
Main Authors: SEUNG II KIM, KIM, Soo-Jung, MYUNG HEE NAM, KIM, Soohyun, HA, Kwon-Soo, OH, Kye-Heon, YOO, Jong-Shin, PARK, Young-Mok
Format: Article
Language:English
Subjects:
ABC transporter
ABC transporters
Acinetobacter - physiology
Acinetobacter lwoffii
Adipates - analysis
Amino Acid Sequence
aniline
Aniline Compounds - pharmacology
Animals
Bacterial Proteins - analysis
Bacteriology
Biological and medical sciences
Chromatography, High Pressure Liquid
Electrophoresis, Gel, Two-Dimensional
Fundamental and applied biological sciences. Psychology
Metabolism. Enzymes
Microbiology
Proteins
Proteome - genetics
Succinic Acid - pharmacology
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Summary:Acinetobacter lwoffii K24 is a soil bacterium that can use aniline as a sole carbon and nitrogen source (by beta-ketoadipate pathway genes (cat genes)) and has two copies of catABC gene separately located on the chromosome. In order to identify aniline-induced proteins, two-dimensional electrophoresis (2-DE) was applied to soluble protein fractions of A. lwoffii K24 cultured in aniline and succinate media. In the range of pH3-10, more than 370 spots were detected on the silver stained gels. Interestingly, more than 20 spots were selectively induced on aniline-cultured bacteria. Twenty-three protein spots of A. lwoffii K24 were analyzed by N-terminal microsequencing and internal microsequencing with in-gel digestion. Of 20 aniline induced protein spots, we identified six beta-ketoadipate pathway genes, one subunit of amino group transfer (putative subunit of aniline oxygenase), malate dehydrogenase, putative ABC transporter, putative hydrolase, HHDD isomerase, and five unknown proteins. Especially in case of two catechol 1,2-dioxygenases (CDI1 and CDI2), more than three isotypes were detected on the 2D gel. This study showed that the proteome analysis of A. lwoffii K24 may be helpful for identification of genes induced by aniline and understanding of their function in the cell.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-001-0075-8