Dihydromyricetin from Ampelopsis grossedentata inhibits melanogenesis through down-regulation of MAPK, PKA and PKC signaling pathways

The inhibitory effects of dihydromyricetin purified from Ampelopsis grossedentata on melanogenesis and its antioxidant characteristics were investigated. Assays of tyrosinase activities and melanin content in B16F10 mouse melanoma cells were carried out spectrophotometrically, and the expression of...

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Published in:Chemico-biological interactions 2016-10, Vol.258, p.166-174
Main Authors: Huang, Huey-Chun, Liao, Chun-Chieh, Peng, Chu-Chun, Lim, Jia-Min, Siao, Jen-Hung, Wei, Chien-Mei, Chen, Chien-Chih, Wu, Chung-Shing, Chang, Tsong-Min
Format: Article
Language:English
Subjects:
Ampelopsis - chemistry
Ampelopsis grossedentata
Animals
Antioxidants - pharmacology
Cell Line, Tumor
Cyclic AMP-Dependent Protein Kinases - metabolism
Dihydromyricetin
Down-Regulation - drug effects
Flavonols - chemistry
Flavonols - pharmacology
Melanin
Melanins - biosynthesis
Melanogenesis
Melanoma, Experimental
Mice
Mitogen-Activated Protein Kinases - metabolism
Protein Kinase C - metabolism
Signal Transduction - drug effects
Tyrosinase
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Summary:The inhibitory effects of dihydromyricetin purified from Ampelopsis grossedentata on melanogenesis and its antioxidant characteristics were investigated. Assays of tyrosinase activities and melanin content in B16F10 mouse melanoma cells were carried out spectrophotometrically, and the expression of melanogenesis-related proteins was determined by Western blotting. The possible signaling pathways involved in dihydromyricetin-mediated depigmentation were also examined using specific protein kinase regulators. The results revealed that dihydromyricetin effectively suppresses intracellular tyrosinase activity and decreases melanin amount in cells. Dihydromyricetin also exhibits antioxidant properties and effectively decreases intracellular reactive oxygen species (ROS) and reactive species (RS) levels. Our results indicated that dihydromyricetin inhibits melanogenesis through its antioxidant properties and by downregulating protein kinase A (PKA), protein kinase C (PKC), and mitogen-activated protein kinases (MAPK) signaling pathways. The present study indicates that dihydromyricetin has the potential to be developed into a depigmentation skin care product. [Display omitted] •Dihydromyricetin inhibits melanogenesis in B16F10 melanoma cells.•Dihydromyricetin decreases the expressions of MC1R, MITF and TRP-1.•Dihydromyricetin effectively decreases intracellular ROS and RS levels.•Dihydromyricetin downregulates MAPK, PKA and PKC pathways.
ISSN:0009-2797
1872-7786
DOI:10.1016/j.cbi.2016.08.023