Immunomodulatory Effects of Psyllium Extract on Helicobacter pylori Interaction With Gastric Epithelial Cells

• Published in: Journal of evidence-based complementary & alternative medicine 2016-10, Vol.21 (4), p.NP18-NP24
• Main Authors: Yakoob, Javed, Jafri, Wasim, Mehmood, Malik Hassan, Abbas, Zaigham, Tariq, Kanwal
• Format: Article
• Language: English
• Subjects: Anti-inflammatory agents; Cell Line, Tumor; Chemical analysis; DNA Fragmentation - drug effects; Gastric Mucosa - cytology; Gastric Mucosa - drug effects; Gastric Mucosa - metabolism; Helicobacter pylori; Helicobacter pylori - drug effects; Host-Pathogen Interactions - drug effects; Humans; Infections; Interleukin-8 - metabolism; NF-kappa B - metabolism; Psyllium - chemistry; Psyllium - pharmacology
• ISSN: 2156-5872; 2156-5899; 2515-690X
• DOI: 10.1177/2156587215611517

Natural plant product Psyllium has anti-inflammatory activity that can modulate the function of cytokines. We determined the effect of Psyllium husk extract on interleukin (IL)-8 and NF-κB secretion by gastric epithelial cells in response to Helicobacter pylori. Human gastric adenocarcinoma cell line (AGS) cells were pretreated with Psyllium extract in different concentrations before H pylori infection. Cell culture supernatant was analyzed for IL-8 and NF-κB by ELISA. RNA from cells was used for real-time polymerase chain reaction for messenger RNA expression of IL-8. Psyllium extract 5 and 10 μg/mL markedly (P < .001) lowered basal IL-8 by 64.71% and 74.51%, respectively, and H pylori–stimulated IL-8 was also (P < .001) lowered by 41.67% and 66.67%, respectively. Psyllium 5 and 10 μg/mL also reduced (P < .0001) cagA-positive H pylori–induced IL-8 mRNA expression by 42.3% and 67.6%, respectively. Psyllium also reduced (P = .0001) NF-κB in response to H pylori strains confirming its role as an anti-inflammatory agent.