Comparative Determination of Anthocyanins, Low Molecular Weight Phenols, and Flavanol Fractions in Vitis vinifera L. cv Carménère Skins and Seeds by Differential Solvent Extraction and High-Performance Liquid Chromatography

Although numerous methods have been used to extract polyphenols from vegetal sources, these procedures have been rarely compared directly. Herein, four maceration and extraction methods for phenolics from Carménère (Vitis vinifera L.) skins and seeds are evaluated. Polyphenols were extracted with mi...

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Bibliographic Details
Published in:Analytical letters 2016-05, Vol.49 (8), p.1127-1142
Main Authors: Izquierdo-Hernández, Alfredo, Peña-Neira, Álvaro, López-Solís, Remigio, Obreque-Slier, Elías
Format: Article
Language:English
Subjects:
Anthocyanins
Concentration (composition)
Extraction methods
grapes
High performance liquid chromatography
HPLC
Methyl alcohol
Phenols
polyphenol
Polyphenols
proanthocyanidin
Seeds
Solvent extraction
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Summary:Although numerous methods have been used to extract polyphenols from vegetal sources, these procedures have been rarely compared directly. Herein, four maceration and extraction methods for phenolics from Carménère (Vitis vinifera L.) skins and seeds are evaluated. Polyphenols were extracted with mixtures of 4:1 (v/v) methanol:water, 4:1 (v/v) acetone:water, 4:1 (v/v) methanol:water, 4:1 (v/v) acetone:water, and 1:9 (v/v) ethanol:water. The extracts were characterized by spectrophotometry for tannins, anthocyanins, total phenols, and color; proanthocyanidin fractionation for monomers, oligomers, and polymers; and high-performance liquid chromatography for anthocyanins and low molecular weight phenols. Seed and skin extracts in methanol/water provided higher polyphenol concentrations compared to the use of acetone/water. Seeds and skins macerated in ethanol/water showed lower concentrations in most polyphenols while seeds and skins that were consecutively macerated in methanol/water and acetone/water showed the highest concentrations of most compounds. In conclusion, solvent polarity, extraction time, and vegetal matrix affected the polyphenol composition of grape seed and skin extracts.
ISSN:0003-2719
1532-236X
DOI:10.1080/00032719.2015.1094661