Static magnetic field reduced exogenous oligonucleotide uptake by spermatozoa using magnetic nanoparticle gene delivery system

Spermatozoa could introduce exogenous oligonucleotides of interest to the oocyte. The most important reason of low efficiency of sperm mediated gene transfer (SMGT) is low uptake of exogenous DNA by spermatozoa. The aim of this study was to evaluate the effects of static magnetic field on exogenous...

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Bibliographic Details
Published in:Journal of magnetism and magnetic materials 2016-03, Vol.402, p.184-189
Main Authors: Katebi, Samira, Esmaeili, Abolghasem, Ghaedi, Kamran
Format: Article
Language:English
Subjects:
Deoxyribonucleic acid
Flow cytometry
Genes
Magnetic fields
Magnetic nanoparticles
Magnetofection
Nanoparticles
Oligonucleotides
SMGT
Spermatozoa
Static magnetic field
Uptakes
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Summary:Spermatozoa could introduce exogenous oligonucleotides of interest to the oocyte. The most important reason of low efficiency of sperm mediated gene transfer (SMGT) is low uptake of exogenous DNA by spermatozoa. The aim of this study was to evaluate the effects of static magnetic field on exogenous oligonucleotide uptake of spermatozoa using magnetofection method. Magnetic nanoparticles (MNPs) associated with the labeled oligonucleotides were used to increase the efficiency of exogenous oligonucleotide uptake by rooster spermatozoa. We used high-field/high-gradient magnet (NdFeB) to enhance and accelerate exogenous DNA sedimentation at the spermatozoa surface. Flow cytometry analysis was performed to measure viability and percentage of exogenous oligonucleotide uptake by sperm. Flow cytometry analysis showed a significant increase in exogenous oligonucleotide uptake by rooster spermatozoa (P
ISSN:0304-8853
DOI:10.1016/j.jmmm.2015.11.057