Hepatitis E virus genotype 3 in mussels (Mytilus galloprovinciallis), Spain

Coastal waters can become contaminated with both human waste from sewage treatment plants and runoff following manure application. Thus, shellfish produced close to land can bioaccumulate enteric viruses of human and animal origin, including zoonotic hepatitis E virus that infect both human and swin...

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Bibliographic Details
Published in:Food microbiology 2016-09, Vol.58, p.13-15
Main Authors: Mesquita, João R., Oliveira, Danielle, Rivadulla, Enrique, Abreu-Silva, Joana, Varela, Miguel F., Romalde, Jesús L., Nascimento, Maria S.J.
Format: Article
Language:English
Subjects:
Animals
Aquaculture
Contamination
Detection
Food Contamination
Food Microbiology
Genotype
Hepatitis
Hepatitis E - transmission
Hepatitis E - virology
Hepatitis E virus
Hepatitis E virus - genetics
Hepatitis E virus - isolation & purification
Hepatitis E virus - physiology
Human
Humans
Mussels
Mytilus
Mytilus - virology
Origins
Phylogeny
RNA, Viral - analysis
Sequence Analysis, DNA
Shellfish
Shellfish - virology
Spain
Swine
Swine Diseases - transmission
Swine Diseases - virology
Viral Load
Viruses
Zoonoses
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Summary:Coastal waters can become contaminated with both human waste from sewage treatment plants and runoff following manure application. Thus, shellfish produced close to land can bioaccumulate enteric viruses of human and animal origin, including zoonotic hepatitis E virus that infect both human and swine. The goal of this study was to evaluate the presence of HEV in shellfish from Galicia (NW Spain), a densely populated region with a strong tradition of swine farming, and one of the most important regions in the world for mussel production. We tested 81 mussel batches by RT-qPCR followed by conventional broad-spectrum nested RT-PCR and phylogenetic analysis. We have obtained 12 positive samples by RT-qPCR (14.81%) with HEV contamination levels ranging from 6.7 × 101 to 8.6 × 104 RNA copies/g digestive tissue. Phylogenetic analysis based on a 330 nt region of the ORF 1 showed that all sequenced isolates belonged to the zoonotic genotype 3 subgenotype e, being closely related to strains of human and swine origin. Results show that shellfish may be a potential route for HEV transmission to humans. •Detection of HEV in shellfish harvested areas is described.•HEV levels ranged from 6.7 × 101 to 8.6 × 104 RNA copies/g digestive tissue.•All sequenced isolates belonged to the zoonotic genotype 3e.•Shellfish constitute a potential route for HEV transmission to humans.
ISSN:0740-0020
1095-9998
DOI:10.1016/j.fm.2016.03.009