Developing a biohybrid lung – sufficient endothelialization of poly-4-methly-1-pentene gas exchange hollow-fiber membranes

Working towards establishing a biohybrid lung with optimized hemocompatibility, this study analyzed the feasibility of establishing flow-resistant endothelium on heparin/albumin coated poly-4-methly-1-pentene hollow fiber gas exchange membranes (PMP-HFs). The seeding efficiency and proliferation of...

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Bibliographic Details
Published in:Journal of the mechanical behavior of biomedical materials 2016-07, Vol.60, p.301-311
Main Authors: Wiegmann, Bettina, von Seggern, Heide, Höffler, Klaus, Korossis, Sotirios, Dipresa, Daniele, Pflaum, Michael, Schmeckebier, Sabrina, Seume, Jörg, Haverich, Axel
Format: Article
Language:English
Subjects:
Activation
Alkenes
Artificial Organs
Bioartificial Lung
Biohybrid lung
Cell Culture Techniques
Cells, Cultured
Contact
Endothelial Cells - cytology
Endothelialization
Feasibility
Gas exchange
Gas exchange membranes
Humans
Lung
Lungs
Markers
Membranes
Monolayers
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Summary:Working towards establishing a biohybrid lung with optimized hemocompatibility, this study analyzed the feasibility of establishing flow-resistant endothelium on heparin/albumin coated poly-4-methly-1-pentene hollow fiber gas exchange membranes (PMP-HFs). The seeding efficiency and proliferation of human cord blood derived endothelial cells (HCBEC) on PMP-HFs were analyzed under static conditions by WST-8 cell proliferation assay and fluorescence microscopy. The HCBEC monolayer integrity under different flow conditions was also assessed. Endothelial-specific phenotype verification, expression activation levels and thrombogenic state markers were quantified by real-time RT-PCR for cell-to-PMP-HF contact under static and dynamic conditions. The results demonstrated the feasibility of establishing a viable, confluent, and flow-resistant endothelial monolayer on the blood-contact surface of PMP-HFs, which maintained a physiological response to TNFα-stimulation and flow conditions. The endothelial phenotype, expression levels of adhesion molecules and thrombogenic state markers were unaffected by cell-to-PMP-HFs contact. These results represent a significant step towards establishing a biohybrid lung.
ISSN:1751-6161
1878-0180
DOI:10.1016/j.jmbbm.2016.01.032