Growth of Campylobacter incubated aerobically in fumarate-pyruvate media or media supplemented with dairy, meat, or soy extracts and peptones

• Published in: Food microbiology 2016-09, Vol.58, p.23-28
• Main Author: Hinton, Arthur
• Format: Article
• Language: English
• Subjects: Aerobic growth; Aerobiosis; Amino Acids - metabolism; Animals; Beef; Campylobacter; Campylobacter - growth & development; Carboxylic Acids - metabolism; Caseins - metabolism; Cattle; Colony Count, Microbial; Culture; Culture Media; Dairy Products; Fumarates - metabolism; Meat; Media; Microbiology; Optical density; Organic acids; Peptide Fragments - metabolism; Peptones; Peptones - metabolism; Pyruvic Acid - metabolism; Red Meat; Soybean Proteins - metabolism
• ISSN: 0740-0020; 1095-9998
• DOI: 10.1016/j.fm.2016.03.010

The ability of Campylobacter to grow aerobically in media supplemented with fumarate-pyruvate or with dairy, meat, or soy extracts or peptones was examined. Optical densities (OD) of Campylobacter cultured in basal media, media supplemented with fumarate-pyruvate or with 1.0, 2.5, 5.0, or 7.5% beef extract was measured. Growth was also compared in media supplemented with other extracts or peptones. Finally, cfu/mL of Campylobacter recovered from basal media or media supplemented with fumarate-pyruvate, casamino acids, beef extract, soytone, or beef extract and soytone was determined. Results indicated that OD of cultures grown in media supplemented with fumarate-pyruvate or with 5.0 or 7.5% beef extract were higher than OD of isolates grown in basal media or media supplemented with lower concentrations of beef extract. Highest OD were produced by isolates grown in media supplemented with beef extract, peptone from meat, polypeptone, proteose peptone, or soytone. Also, more cfu/mL were recovered from media with fumarate-pyruvate, beef extract, soytone, or beef extract-soytone than from basal media or media with casamino acids. Findings indicate that media supplemented with organic acids, vitamins, and minerals and media supplemented with extracts or peptones containing these metabolites can support aerobic growth of Campylobacter. •A novel medium is described that supports Campylobacter growth without the generation of artificial microaerobic atmospheres.•Medium is composed of a basal media supplemented with extracts and peptones from dairy, meat, or soy sources.•Efficacy of peptones and extracts in supporting Campylobacter growth was related to reported concentration of Krebs cycle organic acids and amino acids in these supplements.