Automatic on-line solid-phase extraction with ultra-high performance liquid chromatography and tandem mass spectrometry for the determination of ten antipsychotics in human plasma

An automatic on‐line solid‐phase extraction with ultra‐high performance liquid chromatography and tandem mass spectrometry method was developed for the simultaneous determination of ten antipsychotics in human plasma. The plasma sample after filtration was injected directly into the system without a...

Full description

Saved in:
Bibliographic Details
Published in:Journal of separation science 2016-06, Vol.39 (11), p.2129-2137
Main Authors: Zhong, Qisheng, Shen, Lingling, Liu, Jiaqi, Yu, Dianbao, Li, Simin, Li, Zhiru, Yao, Jinting, Huang, Taohong, Kawano, Shin-ichi, Hashi, Yuki, Zhou, Ting
Format: Article
Language:English
Subjects:
Analytical chemistry
Antipsychotic Agents - blood
Antipsychotics
Automatic on-line system
Automation
Blood plasma
Chromatography
Chromatography, High Pressure Liquid
Extraction
Humans
Liquid chromatography
Mass spectrometry
On-line systems
Plasma
Pretreatment
Psychopharmacology
Psychotropic drugs
Sensitivity and Specificity
Solid Phase Extraction
Tandem Mass Spectrometry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An automatic on‐line solid‐phase extraction with ultra‐high performance liquid chromatography and tandem mass spectrometry method was developed for the simultaneous determination of ten antipsychotics in human plasma. The plasma sample after filtration was injected directly into the system without any pretreatment. A Shim‐pack MAYI‐C8 (G) column was used as a solid‐phase extraction column, and all the analytes were separated on a Shim‐pack XR‐ODS III column with a mobile phase consisting of 0.1% v/v formic acid in water with 5 mM ammonium acetate and acetonitrile. The method features were systematically investigated, including extraction conditions, desorption conditions, the equilibration solution, the valve switching time, and the dilution for column‐head stacking. Under the optimized conditions, the whole analysis procedure took only 10 min. The limits of quantitation were in the range of 0.00321–2.75 μg/L and the recoveries ranged from 75.9 to 122%. Compared with the off‐line ultra‐high performance liquid chromatography and the reported methods, this validated on‐line method showed significant advantages such as minimal pretreatment, shortest analysis time, and highest sensitivity. The results indicated that this automatic on‐line method was rapid, sensitive, and reliable for the determination of antipsychotics in plasma and could be extended to other target analytes in biological samples.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.201600129