Tyrosinase Biosensor for Antioxidants Based on Semiconducting Polymer Support

This study reports sensitive phenolic compounds detection using biosensing electrode constructed by immobilization of tyrosinase in an electrochemically synthesized copolymer based on N‐nonylcarbazole derivatives on a platinum (Pt) electrode. Tyrosinase has been successfully immobilized (electrolyti...

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Bibliographic Details
Published in:Electroanalysis (New York, N.Y.) N.Y.), 2016-06, Vol.28 (6), p.1383-1390
Main Authors: Cabaj, Joanna, Jędrychowska, Agnieszka, Świst, Agnieszka, Sołoducho, Jadwiga
Format: Article
Language:English
Subjects:
Agaricus bisporus
Biosensors
Catalysts
catechol
conducting polymer
Electrodes
enzymatic electrode
Immobilization
phenolic compounds determination
Phenols
Reduction (electrolytic)
Tyrosinase
Voltammetry
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Summary:This study reports sensitive phenolic compounds detection using biosensing electrode constructed by immobilization of tyrosinase in an electrochemically synthesized copolymer based on N‐nonylcarbazole derivatives on a platinum (Pt) electrode. Tyrosinase has been successfully immobilized (electrolytic deposition) on the surface of thin film built of poly[2,7‐bis(selenophene)‐N‐nonylcarbazole] and poly[3,6‐bis(selenophene)‐N‐nonylcarbazole]. A well‐defined reduction current according to the phenolic compounds was observed in cyclic voltammetry, which assigned to the reduction of biocatalytically produced o‐quinones on the electrode surface. The immersion of the tyrosinase‐equipped electrode in solution with substrate generated large catalytic currents easily recorded by cyclic voltammetry. The response of the biosensing arrangement was estimated in the presence of catechol and L‐3,4‐dihydroxyphenylalanine (L‐DOPA). The system exhibits an explicit catalytic activity and the substrates can be amperometrically determined at +0.07 V vs. Ag/AgCl. The activation energy (Ea) of immobilized tyrosinase catalytic reaction was estimated as 24.65 kJ/mol in PBS buffer. The analytical properties of the developed biosensor, such as linear concentration range, sensitivity, detection limit and reproducibility, repeatibility were also evaluated. Considering the fact, that the immobilization policy proved high efficiency, the results suggest that the method for phenoloxidase immobilization has a big capacity of providing high throughput engineering of bioelectronic devices.
ISSN:1040-0397
1521-4109
DOI:10.1002/elan.201500523