Application of a NMR-based untargeted quantitative metabonomic approach to screen for illicit salbutamol administration in cattle

The use of metabonomic methodologies to identify illicit salbutamol administration in cattle has not been previously investigated. In this study, a nuclear magnetic resonance (NMR)-based untargeted quantitative metabonomic approach was applied to discriminate biofluid samples (plasma and urine) obta...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2016-07, Vol.408 (17), p.4777-4783
Main Authors: Tang, Chaohua, Zhang, Kai, Liang, Xiaowei, Zhao, Qingyu, Zhang, Junmin
Format: Article
Language:English
Subjects:
Acetates
Albuterol
Albuterol - administration & dosage
Analytical Chemistry
Animal sciences
Animals
Biochemistry
Biomarkers
Biosensors
Body Fluids - chemistry
Body weight
Cattle
Characterization and Evaluation of Materials
Chemical properties
Chemistry
Chemistry and Materials Science
Discriminant analysis
Drugs
Food Science
Formates
Health aspects
Identification and classification
Illicit
Laboratory Medicine
Magnetic Resonance Spectroscopy - methods
Male
Mass spectrometry
Metabolism
Metabolites
Metabolomics - methods
Methods
Monitoring/Environmental Analysis
NMR
Nuclear magnetic resonance
Nuclear magnetic resonance spectroscopy
Physiology
Plasma
Research Paper
Scientific imaging
Urine
Zoology
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Summary:The use of metabonomic methodologies to identify illicit salbutamol administration in cattle has not been previously investigated. In this study, a nuclear magnetic resonance (NMR)-based untargeted quantitative metabonomic approach was applied to discriminate biofluid samples (plasma and urine) obtained from cattle before and after salbutamol treatment. Six male cattle (265.7 ± 3.9 kg) were fed salbutamol (0.15 mg/kg body weight) for 21 consecutive days. Plasma and urine samples were collected before and after treatment. By the use of targeted profiling, 46 and 43 metabolites in plasma and urine, respectively, were quantified, of which 9 and 11 metabolites were significantly affected ( P  1.0, which implies that these metabolites may serve as potential biomarkers for salbutamol treatment. These findings suggest the potential value of NMR-based untargeted quantitative metabonomic methodologies for plasma and urine analyses as a screening technique for detection of illicit salbutamol usage in cattle.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-016-9571-3