Interface Function and Cefazolin-Adsorption-Release Characteristics of Hydroxyapatite Granules Modified by Supersonic Treatment Techniques

• Published in: Key Engineering Materials 2016-05, Vol.696, p.265-270
• Main Authors: Nakajima, Takehiko, Sakamoto, Michiko, Akazawa, Toshiyuki, Ito, Manabu, Kabir, Md. Arafat, Murata, Masaru, Minamida, Yasuhito
• Format: Article
• Language: English
• Subjects: Amino acids; Biomimetics; Film thickness; Fluids; Granular materials; Granules; Hydroxyapatite; Specific surface
• ISSN: 1013-9826; 1662-9795; 1662-9795
• DOI: 10.4028/www.scientific.net/KEM.696.265

Commercial hydroxyapatite (HAp) porous granules were designed by the supersonic treatment at 120W and 38 kHz for 15 min in parenteral fluids (amino-acids (PF-A), electrolytes and carbohydrates (PF-EC), the mixtures (PF-M)) to form PF-A/HAp, PF-EC/HAp and PF-M/HAp. The modified HAp kept spherical shape and exhibited adhesive aggregates originated from parenteral fluid components on the surface layer. The BET specific surface areas decreased from 39 m2・g-1 to 19-24 m2・g-1. The granules dried at 293K were stirred at 309.5 K in 0.5-2.5 mg・cm-3 CEZ saline solutions. Adsorption isotherms of CEZ for all the granules almost obeyed the Langmuir type-equation. The amounts of CEZ adsorbed on the modified HAp were larger than those on HAp. Regarding CEZ-release characteristics in a biomimetic environment, the CEZ-adsorbed granules were freeze-dried or dried at 293K and they were stirred at 309.5K and pH 7.40 in simulated body fluid (SBF). The CEZ-release efficiencies for granules freeze-dried into SBF were significantly higher than those for granules dried at 293K. The values of granules freeze-dried were 37-43% for PF-A/HAp, 75-83% for PF-EC/HAp, and 56-64% for PF-M/HAp, which were related to the interface function, such as hydrophilicity of surface and multilayer film thickness of water molecule.