Aptazyme–Gold Nanoparticle Sensor for Amplified Molecular Probing in Living Cells

To date, a few of DNAzyme-based sensors have been successfully developed in living cells; however, the intracellular aptazyme sensor has remained underdeveloped. Here, the first aptazyme sensor for amplified molecular probing in living cells is developed. A gold nanoparticle (AuNP) is modified with...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2016-06, Vol.88 (11), p.5981-5987
Main Authors: Yang, Yanjing, Huang, Jin, Yang, Xiaohai, Quan, Ke, Wang, He, Ying, Le, Xie, Nuli, Ou, Min, Wang, Kemin
Format: Article
Language:English
Subjects:
Adenosine triphosphatase
Amplification
Cells
Cells (biology)
Deoxyribonucleic acid
DNA
Fluorescence
Gold
Multiplexing
Nanoparticles
Sensors
Strands
Substrates
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Summary:To date, a few of DNAzyme-based sensors have been successfully developed in living cells; however, the intracellular aptazyme sensor has remained underdeveloped. Here, the first aptazyme sensor for amplified molecular probing in living cells is developed. A gold nanoparticle (AuNP) is modified with substrate strands hybridized to aptazyme strands. Only the target molecule can activate the aptazyme and then cleave and release the fluorophore-labeled substrate strands from the AuNP, resulting in fluorescence enhancement. The process is repeated so that each copy of target can cleave multiplex fluorophore-labeled substrate strands, amplifying the fluorescence signal. Results show that the detection limit is about 200 nM, which is 2 or 3 orders of magnitude lower than that of the reported aptamer-based adenosine triphosphate (ATP) sensors used in living cells. Furthermore, it is demonstrated that the aptazyme sensor can readily enter living cells and realize intracellular target detection.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.6b00999