Removal efficiency of MIEX registered pretreatment on typical proteins and amino acids derived from Microcystis aeruginosa

Dissolved organic nitrogen (DON) is now considered as one of the most important precursors of nitrogenous disinfection byproducts (N-DBPs), and algal cells are the main source of DON in eutrophic water sources. In this study, typical forms of DON in Microcystis aeruginosacells were analyzed and the...

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Bibliographic Details
Published in:RSC advances 2016-06, Vol.6 (65), p.60869-60876
Main Authors: Liu, Cheng, He, Siyuan, Sun, Zhehao, Wang, Jie, Chen, Wei
Format: Article
Language:English
Subjects:
Algae
Amino acids
Aspartic acid
Microcystis
Microcystis aeruginosa
Phycocyanin
Pretreatment
Proteins
Tryptophan
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Summary:Dissolved organic nitrogen (DON) is now considered as one of the most important precursors of nitrogenous disinfection byproducts (N-DBPs), and algal cells are the main source of DON in eutrophic water sources. In this study, typical forms of DON in Microcystis aeruginosacells were analyzed and the removal efficiency of magnetic ion exchange resin (MIEX registered ) treatment on typical forms of DON and the factors influencing it were also investigated. About 185 proteins and 13 amino acids were found in the intracellular organic matter (IOM) of M. aeruginosacells. The isoelectric points (PIs) of these proteins were mostly acidic, and phycocyanin was the principal protein with a concentration of about 0.673 mg L super(-1). In addition, high levels of aspartic acid, histidine, tryptophan, and asparagine were also noted, which presented higher potential to produce N-DBPs during the chlorination process. The removal efficiencies of MIEX registered on various amino acids and phycocyanin were quite different primarily because of their different PIs and molecular properties. The pH was noted to be the main factor affecting the removal efficiency, and common inorganic anions also had obvious influence on the removal of amino acids and phycocyanin the following order: SO sub(4) super(2-) > Cl super(-) > HCO sub(3) super(-). The proteins in the IOM were effectively removed by MIEX registered and only 50 proteins were found in the spectrum of two-dimensional electrophoresis. Thus, coagulation could be significantly enhanced by MIEX registered pretreatment, which is one of the good alternatives to control typical DON in IOM.
ISSN:2046-2069
DOI:10.1039/c6ra11109b