A Method for Detecting Circulating Tumor Cells Based on the Measurement of Single-Cell Metabolism in Droplet-Based Microfluidics

The number of circulating tumor cells (CTCs) in blood is strongly correlated with the progress of metastatic cancer. Current methods to detect CTCs are based on immunostaining or discrimination of physical properties. Herein, a label‐free method is presented exploiting the abnormal metabolic behavio...

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Bibliographic Details
Published in:Angewandte Chemie 2016-07, Vol.128 (30), p.8723-8726
Main Authors: Del Ben, Fabio, Turetta, Matteo, Celetti, Giorgia, Piruska, Aigars, Bulfoni, Michela, Cesselli, Daniela, Huck, Wilhelm T. S., Scoles, Giacinto
Format: Article
Language:English
Subjects:
Blood
Cancer
Chemistry
Droplets
Krebs
Metabolism
Metastasis
Microfluidics
Mikrofluidik
Physical properties
Pikotröpfchen
Secretions
Stoffwechsel
Tumors
Zirkulierende Tumorzellen
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Summary:The number of circulating tumor cells (CTCs) in blood is strongly correlated with the progress of metastatic cancer. Current methods to detect CTCs are based on immunostaining or discrimination of physical properties. Herein, a label‐free method is presented exploiting the abnormal metabolic behavior of cancer cells. A single‐cell analysis technique is used to measure the secretion of acid from individual living tumor cells compartmentalized in microfluidically prepared, monodisperse, picoliter (pL) droplets. As few as 10 tumor cells can be detected in a background of 200 000 white blood cells and proof‐of‐concept data is shown on the detection of CTCs in the blood of metastatic patients. Ein veränderter Stoffwechsel ist ein Erkennungsmerkmal von Krebszellen. Die Unterschiede zu gesunden Zellen sind besonders gut in winzigen pL‐Tröpfchen, die einzelne Zellen enthalten, erkennbar. Da Krebszellen den pH‐Wert ihrer Mikroumgebung binnen Minuten verändern, können pH‐Messungen für einzelne Tröpfchen zirkulierende Tumorzellen (CTCs) in Patientenproben nachweisen. WBC=weißes Blutkörperchen, Lac=Lactat.
ISSN:0044-8249
1521-3757
DOI:10.1002/ange.201602328