Clastogenicity of pentachlorophenol, 2,4-D and butachlor evaluated by Allium root tip test

The meristematic mitotic cells of Allium cepa is an efficient cytogenetic material for chromosome aberration assay on environmental pollutants. For assessing genotoxicity of pentachlorophenol (PCP), 2,4-dichlorophenoxyacetic acid (2,4-D) and 2-chloro-2,6-diethyl- N-(butoxymethyl) acetanilide (butach...

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Bibliographic Details
Published in:Mutation research. Genetic toxicology and environmental mutagenesis 2002-02, Vol.514 (1), p.105-113
Main Authors: Ateeq, Bushra, Abul Farah, M, Niamat Ali, M, Ahmad, Waseem
Format: Article
Language:English
Subjects:
Allium
Allium cepa
Biological and medical sciences
butachlor
Chromosome aberration
Fundamental and applied biological sciences. Psychology
Herbicides
Molecular and cellular biology
Molecular genetics
Mutagenesis. Repair
Toxicity assay
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Summary:The meristematic mitotic cells of Allium cepa is an efficient cytogenetic material for chromosome aberration assay on environmental pollutants. For assessing genotoxicity of pentachlorophenol (PCP), 2,4-dichlorophenoxyacetic acid (2,4-D) and 2-chloro-2,6-diethyl- N-(butoxymethyl) acetanilide (butachlor), 50% effective concentration (EC 50), c-mitosis, stickiness, chromosome breaks and mitotic index (MI) were used as endpoints of genotoxicity. EC 50 values for PCP and butachlor are 0.73 and 5.13 ppm, respectively. 2,4-D evidently induced morphological changes at higher concentrations. Some changes like crochet hooks, c-tumours and broken roots were unique to 2,4-D at 5–20 ppm. No such abnormalities were found in PCP and butachlor treated groups, however, root deteriorated and degenerated at higher concentrations (
ISSN:1383-5718
1879-3592
DOI:10.1016/S1383-5718(01)00327-8