Fluorescent probe for detection of bacteria: conformational trigger upon bacterial reduction of an azo bridge

Using biomimetic chemical reduction or Clostridium perfringens cell extract containing azoreductase, the dimer-fluorescent probe 2,4-O-bisdansyl-6,7-diazabicyclooct-6-ene, which possesses a conformationally constrained cis-azo bridge, is reduced to the tetra-equatorial 2,4-O-bisdansyl-cyclohexyl-3,5...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2012-05, Vol.48 (51), p.6393-6395
Main Authors: Rattray, Nicholas J W, Zalloum, Waleed A, Mansell, David, Latimer, Joe, Schwalbe, Carl H, Blake, Alexander J, Bichenkova, Elena V, Freeman, Sally
Format: Article
Language:English
Subjects:
Bacteria
Clostridium
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Summary:Using biomimetic chemical reduction or Clostridium perfringens cell extract containing azoreductase, the dimer-fluorescent probe 2,4-O-bisdansyl-6,7-diazabicyclooct-6-ene, which possesses a conformationally constrained cis-azo bridge, is reduced to the tetra-equatorial 2,4-O-bisdansyl-cyclohexyl-3,5-bisammonium salt which exhibits fluorescence indicative of a dansyl monomer.
ISSN:1359-7345
1364-548X
DOI:10.1039/c2cc32521g