Taenia solium metacestode fasciclin‐like protein is reactive with sera of chronic neurocysticercosis

• Published in: Tropical medicine & international health 2014-06, Vol.19 (6), p.719-725
• Main Authors: Bae, Young‐An, Yeom, Joon‐Sup, Wang, Hu, Kim, Seon‐Hee, Ahn, Chun‐Seob, Kim, Jin‐Taek, Yang, Hyun‐Jong, Kong, Yoon
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biomarkers; chronic stage; Cysticercosis; Diseases caused by cestodes; estadío crónico inactivo; fasciclin‐like protein; General aspects; Helminthic diseases; Infectious diseases; Medical sciences; metacéstodo Taenia solium; métacestode de Taenia solium; neurocisticercosis; neurocysticercose; neurocysticercosis; Neurological disorders; Parasites; Parasitic diseases; Proteins; Proteomics; proteína similar a la fasciclina; protéines semblable à la fascicline; serodiagnosis; serodiagnóstico; stade chronique inactif; sérodiagnostic; Taenia solium metacestode
• ISSN: 1360-2276; 1365-3156
• DOI: 10.1111/tmi.12302

Objectives Neurocysticercosis (NC), an infection of the central nervous system with Taenia solium metacestodes (TsM), invokes a formidable neurological disease. A bundle of antigens is applicable for serodiagnosis of active cases, while they demonstrate fairly low reactivity against sera of chronic NC. Identification of sensitive biomarkers for chronic NC is critical for appropriate management of patients. Methods Proteome analysis revealed several isoforms of 65‐ and 83‐kDa TsM fasciclin‐like proteins (TsMFas) to be highly reactive with sera of chronic NC. A cDNA encoding one of the 83‐kDa TsMFas (TsMFas1) was isolated from a cDNA library. We expressed a recombinant protein (rTsMFas1) and evaluated its diagnostic potential employing sera from chronic NC (n = 80), tissue‐invasive cestodiases (n = 169) and trematodiases (n = 80) and those of normal controls (n = 50). Results Secretory TsMFas1 was composed of 766 amino acid polypeptide and harboured fasciclin and fasciclin‐superfamily domains. The protein was constitutively expressed in metacestode and adult stages, with preferential locality in the scolex. Bacterially expressed rTsMFas1 exhibited 78.8% sensitivity (63/80 cases) and 93% specificity (278/299 samples) in diagnosing chronic NC. Some cross‐reactivity was observed with sera of cystic echinococcosis (10/56, 17.8%) and sparganosis (4/50, 8%). Positive and negative predictive values were 75% and 95.5%, respectively. Conclusion TsM fasciclin‐like protein may be useful for differential diagnosis of chronic NC in clinical settings, especially where both NC and other infectious cerebral granulomatoses are prevalent. Objectifs La neurocysticercose (NC), une infection du système nerveux central par les métacestodes du Taenia solium (TsM), invoque une maladie neurologique redoutable. Une panoplie d'antigènes sont applicables pour le diagnostique sérologique des cas actifs, ils démontrent cependant une réactivité assez faible avec les sérums de NC chronique. L'identification de biomarqueurs sensibles pour la NC inactive est essentielle pour une prise en charge appropriée des patients. Méthodes Une analyse protéomique a révélé plusieurs isoformes de 65 et 83 kDa du TsM, semblables à la protéine fascicline (TsMFas) très réactives avec des sérums de NC inactive. Un ADNc codant pour l'une des 83 kDa de TsMFas (TsMFas1) a été isolé à partir d'une banque d'ADNc. Nous avons exprimé une protéine recombinante (rTsMFas1) et évalué son potentiel diagnostic en utilisan