Isolation and identification of cyclic lipopeptides from Paenibacillus ehimensis, strain IB-X-b

•Procedure of isolation of antifungal compounds from P. ehimensis is developed.•Antifungal compounds of P. ehimensis include two fractions of cyclic lipopeptides.•Main fraction contains bacillomycin L and fengycin/plipastatin/agrastatin families.•Other group comprises depsipeptides belonging to poly...

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Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2014-12, Vol.973, p.9-16
Main Authors: Aktuganov, Gleb, Jokela, Jouni, Kivelä, Henri, Khalikova, Elvira, Melentjev, Alexander, Galimzianova, Nailia, Kuzmina, Lyudmila, Kouvonen, Petri, Himanen, Juha-Pekka, Susi, Petri, Korpela, Timo
Format: Article
Language:English
Subjects:
Amino acids
Antifungal compounds
Biocontrol
Biomedical materials
Chromatography
Culture
Cyclic lipopeptides
Fatty acids
Homology
LC–MS
Microbial antagonism
Paenibacillus
Paenibacillus ehimensis
Purification
Residues
Strain
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Summary:•Procedure of isolation of antifungal compounds from P. ehimensis is developed.•Antifungal compounds of P. ehimensis include two fractions of cyclic lipopeptides.•Main fraction contains bacillomycin L and fengycin/plipastatin/agrastatin families.•Other group comprises depsipeptides belonging to polypeptine family. Antifungal lipopeptides produced by an antagonistic bacterium, Paenibacillus ehimensis strain IB-X-b, were purified and analyzed. The acetone extract of the culture supernatant contained an antifungal amphiphilic fraction stainable with ninhydrin on thin layer chromatography. The fraction was further purified with water–methanol extraction followed by a chromatography on a C18-support. The analysis with LC–MS showed presence of two main series of homologous compounds, family of depsipeptides containing a hydroxy fatty acid, three 2,4-diaminobutyric acid (Dab) residues, five hydrophobic amino acids and one Ser/Thr residue, and cyclic lipopeptides of bacillomycin L and fengycin/plipastatin/agrastatin families. The prevailing compounds in this group are bacillomycin L-C15, fengycin/plipastatin A-C16 together with their homologues responsible for the majority of fungal growth inhibition by P. ehimensis IB-X-b.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2014.09.042