Modeling the fate of antibiotic resistance genes and class 1 integrons during thermophilic anaerobic digestion of municipal wastewater solids

• Published in: Applied microbiology and biotechnology 2016-02, Vol.100 (3), p.1437-1444
• Main Authors: Burch, Tucker R, Sadowsky, Michael J, LaPara, Timothy M
• Format: Article
• Language: English
• Subjects: anaerobic digesters; Anaerobic digestion; Antibiotic resistance; Antibiotics; Bacteria; Biogas; Biomedical and Life Sciences; Biotechnology; disinfection; Drug resistance; Drug resistance in microorganisms; Environmental Biotechnology; fluoroquinolones; Gene expression; Genes; Genetic aspects; Health aspects; Integrons; Life Sciences; Medical laboratories; methanogens; Microbial Genetics and Genomics; Microbiology; Municipal solid waste; Municipal wastewater; Observations; pollutants; Properties; Public health; quantitative polymerase chain reaction; Refuse as fuel; ribosomal RNA; Solids; Studies; Temperature; tetracycline; Wastewater; Water treatment
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-015-7043-x

This study investigated the use of thermophilic anaerobic digestion for removing antibiotic resistance genes (ARGs) from residual municipal wastewater solids. Four laboratory-scale anaerobic digesters were operated in 8-day batch cycles at temperatures of 40, 56, 60, and 63 °C. Two tetracycline resistance genes (tet(W) and tet(X)), a fluoroquinolone resistance gene (qnrA), the integrase gene of class 1 integrons (intI1), 16S rRNA genes of all Bacteria, and 16S rRNA genes of methanogens were quantified using real-time quantitative PCR. ARG and intI1 quantities decreased at all temperatures and were described well by a modified form of the Collins-Selleck disinfection kinetic model. The magnitudes of Collins-Selleck kinetic parameters were significantly greater at thermophilic temperatures compared to 40 °C, but few statistically significant differences were observed among these parameters for the thermophilic anaerobic digesters. This model allows for the direct comparison of different operating conditions (e.g., temperature) on anaerobic digestion performance in mitigating the quantity of ARGs in wastewater solids and could be used to design full-scale anaerobic digesters to specifically treat for ARGs as a “pollutant” of concern.