A Combinatorial effect of carboxymethyl cellulose based scaffold and microRNA-15b on osteoblast differentiation

• Published in: International journal of biological macromolecules 2016-12, Vol.93 (Pt B), p.1457-1464
• Main Authors: Vimalraj, S., Saravanan, S., Vairamani, M., Gopalakrishnan, C., Sastry, T.P., Selvamurugan, N.
• Format: Article
• Language: English
• Subjects: Animals; Bone Regeneration; Bone Substitutes; Carboxylmethyl cellulose/Zn doped nano-hydroxyapatite/ascorbic acid; Carboxymethylcellulose Sodium - pharmacology; Cell Differentiation; Cell Survival; Cells, Cultured; Materials Testing; Mesenchymal stem cells; Mesenchymal Stromal Cells - physiology; Mice; MicroRNAs - physiology; miR-15b; Osteoblasts - physiology; Tissue Scaffolds - chemistry
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2015.12.083

The present study was aimed to synthesize and characterize a bio-composite scaffold containing carboxymethyl cellulose (CMC), zinc doped nano-hydroxyapatite (Zn-nHAp) and ascorbic acid (AC) for bone tissue engineering applications. The fabricated bio-composite scaffold was characterized by SEM, FT-IR and XRD analyses. The ability of scaffold along with a bioactive molecule, microRNA-15b (miR-15b) for osteo-differentiation at cellular and molecular levels was determined using mouse mesenchymal stem cells (mMSCs). miR-15b acts as posttranscriptional gene regulator and regulates osteoblast differentiation. The scaffold and miR-15b were able to promote osteoblast differentiation; when these treatments were combined together on mMSCs, there was an additive effect on promotion of osteoblast differentiation. Thus, it appears that the combination of CMC/Zn-nHAp/AC scaffold with miR-15b would provide more efficient strategy for treating bone related defects and bone regeneration.