Molecular identification of Bigeyes (Perciformes, Priacanthidae) from Indian waters

Thirty-five individuals of six priacanthid fish species were sampled from different localities along the coast of India covering the Arabian Sea and Bay of Bengal. The partial sequence of 16S rRNA and cytochrome oxidase subunit I (COI) genes were analyzed for species identification and phylogenetic...

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Bibliographic Details
Published in:Mitochondrial DNA. Part A. DNA mapping, sequencing, and analysis sequencing, and analysis, 2016-11, Vol.27 (6), p.4638-4642
Main Authors: K. K, Bineesh, A, Gopalakrishnan, J. K, Jena, V. S, Basheer, C, Mohitha, N, Vineesh, M, Joselet, Pillai, N. G. K.
Format: Article
Language:English
Subjects:
16S rRNA
Animals
Base Composition
Bigeyes
COI
DNA barcoding
DNA, Mitochondrial - isolation & purification
DNA, Mitochondrial - metabolism
Electron Transport Complex IV - chemistry
Electron Transport Complex IV - classification
Electron Transport Complex IV - genetics
Electron Transport Complex IV - metabolism
Genome, Mitochondrial
India
Indian waters
Perciformes - classification
Perciformes - genetics
Phylogeny
RNA, Ribosomal, 16S - chemistry
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
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Summary:Thirty-five individuals of six priacanthid fish species were sampled from different localities along the coast of India covering the Arabian Sea and Bay of Bengal. The partial sequence of 16S rRNA and cytochrome oxidase subunit I (COI) genes were analyzed for species identification and phylogenetic relationship among the Indian priacanthids (Priacanthus hamrur, P. prolixus, P. blochii, P. sagittarius, Cookeolus japonicus, and Pristigenys refulgens). The intraspecies genetic distance ranged from 0.000 to 0.002, while distances varied from 0.008 to 0.157 interspecies based on 16S sequences. Using COI data analysis, the intraspecies genetic distance ranged from 0.000 to 0.005, while interspecies distances varied from 0.009 to 0.108. Several sequences labeled Priacanthus hamrur in GenBank are shown to be P. prolixus. We also observed cryptic speciation in Heteropriacanthus cruentatus. Partial sequences of 16S rRNA and COI genes provided phylogenetic information to distinguish thirteen species of priacanthids, indicating the usefulness of molecular markers in species identification.
ISSN:2470-1394
2470-1408
DOI:10.3109/19401736.2015.1101593