Interaction between thyrocytes and adipose tissue in vitro

Adipose tissue (AT)‐thyrocyte interaction is largely unknown. Here we described the interaction in a co‐culture system, in which thyrocytes were cultured on AT fragment (ATF)‐embedded collagen gel, using electron microscopy, immunocytochemistry, real‐time reverse transcription–polymerase chain react...

Full description

Saved in:
Bibliographic Details
Published in:Pathology international 2016-03, Vol.66 (3), p.148-157
Main Authors: Yamamoto, Mihoko, Uchihashi, Kazuyoshi, Aoki, Shigehisa, Koike, Eisuke, Kakihara, Nahoko, Toda, Shuji
Format: Article
Language:English
Subjects:
adipokines
Adiponectin - genetics
Adiponectin - metabolism
adipose tissue
Adipose Tissue - cytology
Adipose Tissue - physiology
Animals
Apoptosis
Cell Differentiation
Cell Proliferation
Cells, Cultured
Coculture Techniques
Collagen
Humans
Hypertrophy
interaction
Leptin - genetics
Leptin - metabolism
lipid deposition
Lipid Metabolism
Male
PAX8 Transcription Factor - genetics
PAX8 Transcription Factor - metabolism
Rats, Wistar
RNA, Messenger - genetics
thyrocytes
Thyroglobulin - metabolism
Thyroid Epithelial Cells - cytology
Thyroid Epithelial Cells - physiology
Thyrotropin - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Adipose tissue (AT)‐thyrocyte interaction is largely unknown. Here we described the interaction in a co‐culture system, in which thyrocytes were cultured on AT fragment (ATF)‐embedded collagen gel, using electron microscopy, immunocytochemistry, real‐time reverse transcription–polymerase chain reaction (RT–PCR) and enzyme‐linked immunosorbent assay (ELISA). ATFs promoted the hypertrophy, polarization and lipid accumulation of thyrocytes. ATFs did not affect the growth of thyroyctes, and inhibited their apoptosis. ATFs increased the protein expression of thyroglobulin (Tg) and paired box gene 8 (PAX8) in thyrocytes. In turn, thyrocytes decreased the concentration of leptin and adiponectin, and increased the expression of these mRNAs in ATFs. Thyrotropin (TSH) enhanced the ATF‐induced nuclear hypertrophy and Tg protein expression in thyrocytes, while TSH enhanced the thyrocyte‐induced expression of leptin and adiponectin mRNAs in ATFs. Finally, leptin promoted the hypertrophy and Tg protein expression in thyrocytes. TSH enhanced these leptin‐induced effects. The data indicate an active interaction between thyrocytes and AT, suggesting that (i) ATFs may serve to regulate the morphology, survival and differentiation of thyrocytes probably through lipid accumulation partly in a TSH‐synergistic way; (ii) thyrocytes may affect adipokine production from ATFs in a TSH‐independent manner; and (3) leptin may be related to the hypertrophy and differentiation of thyrocytes in a TSH‐synergistic way.
ISSN:1320-5463
1440-1827
DOI:10.1111/pin.12387