Biomimetic hydrogel loaded with silk and l-proline for tissue engineering and wound healing applications

The aim of this article was to develop silk protein (SF) and l-proline (LP) loaded chitosan-(CS) based hydrogels via physical cross linking for tissue engineering and wound healing applications. Silk fibroin, a biodegradable and biocompatible protein, and l-proline, an important imino acid that is r...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2017-08, Vol.105 (6), p.1401-1408
Main Authors: Thangavel, Ponrasu, Ramachandran, Balaji, Kannan, Ramya, Muthuvijayan, Vignesh
Format: Article
Language:English
Subjects:
Absorbable Implants
Animals
Biocompatibility
Biodegradability
Biodegradation
Biomedical materials
Biomimetic Materials - chemistry
Biomimetics
Cell Adhesion
Cell adhesion & migration
Cell Proliferation
Cell Survival
Chitosan
Collagen
Crosslinking
Degradation
Fibroins - chemistry
Hydrogels
Hydrogels - chemistry
In vitro methods and tests
Lysozyme
Materials research
Materials science
Materials Testing
Mice
NIH 3T3 Cells
Proline
Proline - chemistry
Regeneration
Silk
Silk fibroin
Surface stability
Thermal stability
Tissue Engineering
Wound Healing
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Summary:The aim of this article was to develop silk protein (SF) and l-proline (LP) loaded chitosan-(CS) based hydrogels via physical cross linking for tissue engineering and wound healing applications. Silk fibroin, a biodegradable and biocompatible protein, and l-proline, an important imino acid that is required for collagen synthesis, were added to chitosan to improve the wound healing properties of the hydrogel. Characterization of these hydrogels revealed that CS/SF/LP hydrogels were blended properly and LP incorporated hydrogels showed excellent thermal stability and good surface morphology. Swelling study showed the water holding efficiency of the hydrogels to provide enough moisture at the wound surface. In vitro biodegradation results demonstrated that the hydrogels had good degradation rate in PBS with lysozyme. LP loaded hydrogels showed approximately a twofold increase in antioxidant activity. In vitro cytocompatibility studies using NIH 3T3 L1 cells showed increased cell viability (p 
ISSN:1552-4973
1552-4981
DOI:10.1002/jbm.b.33675