The Effects of Losartan in Preserving the Structural Integrity of Decellularized Small Diameter Vascular Allograft Conduit Implants In Vivo

Decellularization is a proposed method of preparing nonautologous biological arterial vascular scaffolding; however, the fate of the supporting medial elastic fiber, which is important in preserving the vascular structural integrity, is uncertain. The influence of losartan on preserving the medial e...

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Bibliographic Details
Published in:Artificial organs 2017-01, Vol.41 (1), p.98-106
Main Authors: Lee, Seung Hyun, Lee, Byoung Wook, Kim, Seong Who, Choo, Suk Jung
Format: Article
Language:English
Subjects:
Allografts
Animals
Antihypertensive Agents - pharmacology
Aorta, Abdominal - drug effects
Aorta, Abdominal - transplantation
Aorta, Abdominal - ultrastructure
Biomechanical Phenomena - drug effects
Bioprosthesis
Blood Vessel Prosthesis
Elasticity - drug effects
Female
Losartan
Losartan - pharmacology
Medial elastic fiber integrity
Rats, Sprague-Dawley
Rodents
Small diameter vascular conduits
Tissue Scaffolds - chemistry
Transplantation, Homologous
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Summary:Decellularization is a proposed method of preparing nonautologous biological arterial vascular scaffolding; however, the fate of the supporting medial elastic fiber, which is important in preserving the vascular structural integrity, is uncertain. The influence of losartan on preserving the medial elastic fiber integrity in decellularized small diameter vascular conduits (SDVC) was investigated. Decellularized infrarenal abdominal aortic allografts were implanted in Sprague‐Dawley rats treated either with (study rats, n = 6) or without oral losartan (control rats, n = 6) and graded 8 weeks later according to a remodeling scoring system (1‐mild, 2‐moderate, 3‐severe) which we devised based on the intimal hyperplasia degree, morphologic changes, and elastic fiber fragmentation of the conduits. DAPI immunohistochemistry analysis was performed in 47 (25 decellularization only and 22 losartan treatment) cross‐sectional slide specimens. The losartan versus decellularization only SDVC showed a significantly lower medial elastic fragmentation score (1.32 vs. 2.24, P < 0.001), superior medial layer preservation, and relatively more normal appearing intimal cellular morphology. The results suggested rats receiving decellularized SDVCs treated with losartan may yield superior medial layer elastic fiber preservation.
ISSN:0160-564X
1525-1594
DOI:10.1111/aor.12686