European canine lymphoma network consensus recommendations for reporting flow cytometry in canine hematopoietic neoplasms

• Published in: Cytometry. Part B, Clinical cytometry Clinical cytometry, 2017-09, Vol.92 (5), p.411-419
• Main Authors: Comazzi, S., Avery, P. R., Garden, O. A., Riondato, F., Rütgen, B., Vernau, W.
• Format: Article
• Language: English
• Subjects: Aberration; Animals; Consensus; Cytometry; Diagnosis; Diagnostic systems; dog; Dog Diseases - diagnosis; Dogs; European canine lymphoma network; Feedback; Flow cytometry; Flow Cytometry - methods; Fluorescence; guidelines; Hematologic Neoplasms - diagnosis; Hematologic Neoplasms - veterinary; Humans; Immunophenotyping - methods; Institutions; International cooperation; Leukemia; Leukemia - diagnosis; Lymphoma; Lymphoma - pathology; Neoplasms; Standardization; Tumors; Veterinary medicine
• ISSN: 1552-4949; 1552-4957
• DOI: 10.1002/cyto.b.21382

Background Flow cytometry (FC) is assuming increasing importance in diagnosis in veterinary oncology. The European Canine Lymphoma Network (ECLN) is an international cooperation of different institutions working on canine lymphoma diagnosis and therapy. The ECLN panel of experts on FC has defined the issue of reporting FC on canine lymphoma and leukemia as their first hot topic, since a standardized report that includes all the important information is still lacking in veterinary medicine. Methods The flow cytometry panel of the ECLN started a consensus initiative using the Delphi approach. Clinicians were considered the main target of FC reports. A panel of experts in FC was interrogated about the important information needed from a report. Results Using the feedback from clinicians and subsequent discussion, a list of information to be included in the report was made, with four different levels of recommendation. The final report should include both a quantitative part and a qualitative or descriptive part with interpretation of the salient results. Other items discussed included the necessity of reporting data regarding the quality of samples, use of absolute numbers of positive cells, cutoff values, the intensity of fluorescence, and possible aberrant patterns of antigen expression useful from a clinical point of view. Conclusion The consensus initiative is a first step toward standardization of diagnostic approach to canine hematopoietic neoplasms among different institutions and countries. This harmonization will improve communication and patient care and also facilitate the multicenter studies necessary to further our knowledge of canine hematopoietic neoplasms. © 2016 International Clinical Cytometry Society