Cre Recombinase and Other Tyrosine Recombinases

Tyrosine-type site-specific recombinases (T-SSRs) have opened new avenues for the predictable modification of genomes as they enable precise genome editing in heterologous hosts. These enzymes are ubiquitous in eubacteria, prevalent in archaea and temperate phages, present in certain yeast strains,...

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Bibliographic Details
Published in:Chemical reviews 2016-10, Vol.116 (20), p.12785-12820
Main Authors: Meinke, Gretchen, Bohm, Andrew, Hauber, Joachim, Pisabarro, M. Teresa, Buchholz, Frank
Format: Article
Language:English
Subjects:
Deoxyribonucleic acid
DNA
DNA - metabolism
DNA Nucleotidyltransferases - chemistry
DNA Nucleotidyltransferases - metabolism
Enzymes
Eukaryotes
Genetics
Genomes
Integrases - chemistry
Integrases - metabolism
Protein Conformation
Tyrosine - metabolism
Yeast
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Summary:Tyrosine-type site-specific recombinases (T-SSRs) have opened new avenues for the predictable modification of genomes as they enable precise genome editing in heterologous hosts. These enzymes are ubiquitous in eubacteria, prevalent in archaea and temperate phages, present in certain yeast strains, but barely found in higher eukaryotes. As tools they find increasing use for the generation and systematic modification of genomes in a plethora of organisms. If applied in host organisms, they enable precise DNA cleavage and ligation without the gain or loss of nucleotides. Criteria directing the choice of the most appropriate T-SSR system for genetic engineering include that, whenever possible, the recombinase should act independent of cofactors and that the target sequences should be long enough to be unique in a given genome. This review is focused on recent advancements in our mechanistic understanding of simple T-SSRs and their application in developmental and synthetic biology, as well as in biomedical research.
ISSN:0009-2665
1520-6890
DOI:10.1021/acs.chemrev.6b00077