Resveratrol Inhibits Diabetic-Induced Müller Cells Apoptosis through MicroRNA-29b/Specificity Protein 1 Pathway

• Published in: Molecular neurobiology 2017-08, Vol.54 (6), p.4000-4014
• Main Authors: Zeng, Kaihong, Wang, Yuan, Yang, Na, Wang, Duozi, Li, Suping, Ming, Jian, Wang, Jing, Yu, Xuemei, Song, Yi, Zhou, Xue, Deng, Bo, Wu, Xiaona, Huang, Lujiao, Yang, Yongtao
• Format: Article
• Language: English
• Subjects: Animals; Annexin V; Apoptosis; Apoptosis - drug effects; Assaying; BAX protein; Bcl protein; Bcl-2 protein; bcl-2-Associated X Protein - metabolism; Biomedical and Life Sciences; Biomedicine; Biotin; Blood Glucose - metabolism; Caspase; Caspase 3 - metabolism; Caspase-3; Cell Biology; Cells, Cultured; Chains (polymeric); Diabetes; Diabetes mellitus; Diabetes Mellitus, Experimental - blood; Diabetes Mellitus, Experimental - genetics; Diabetes Mellitus, Experimental - pathology; Diabetic retinopathy; DNA nucleotidylexotransferase; Ependymoglial Cells - drug effects; Ependymoglial Cells - metabolism; Ependymoglial Cells - pathology; Fructosamine - blood; Gene expression; Gene Expression Regulation - drug effects; Glucose; Immunofluorescence; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA; Neurobiology; Neurology; Neurosciences; Polyimide resins; Polymerase chain reaction; Proteins; Rats; Rats, Sprague-Dawley; Resveratrol; Retina; Retina - drug effects; Retina - pathology; Retinal cells; Retinopathy; RNA, Messenger - genetics; RNA, Messenger - metabolism; Rodents; Sp1 protein; Sp1 Transcription Factor - genetics; Sp1 Transcription Factor - metabolism; Staining; Stilbenes - pharmacology; Streptozocin; Western blotting
• ISSN: 0893-7648; 1559-1182
• DOI: 10.1007/s12035-016-9972-5

The aim of this study was to evaluate the anti-apoptosis effects of resveratrol (RSV) on diabetic rats retinal Müller cells in vivo and in vitro and to further investigate the roles of microRNA-29b (miR-29b)/specificity protein 1 (SP1) in the anti-apoptosis mechanism of RSV. Retina was obtained from normal and diabetic rats with or without RSV (5 and 10 mg/kg/day) treatments at 1–7 months. TdT-mediated dUTP-biotin nick end labeling (TUNEL) and Annexin V/PI staining were used to detect apoptosis. Immunofluorescence was used to assess distribution of SP1 in retina. MiR-29b and SP1 messenger RNA (mRNA) expression was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). SP1, Bax, and bcl-2 protein expression was evaluated by western blotting. Caspase-3 activity was detected by assay kit. Our study showed that the TUNEL-positive cells were mainly localized in the inner nuclear layer (INL) of retina and RSV administration effectively suppressed streptozotocin (STZ)-induced apoptosis of retinal cells in INL in vivo ( P