The applications of CRISPR screen in functional genomics

CRISPR technology has rapidly changed the face of biological research, such that precise genome editing has now become routine for many labs within several years of its initial development. CRISPR/Cas9 (Clustered Regularly Interspace Short Palindromic Repeat/CRISPR-associated nuclease 9) gene editin...

Full description

Saved in:
Bibliographic Details
Published in:Briefings in functional genomics 2017-01, Vol.16 (1), p.34-37
Main Authors: Qi, Xiaolong, Zhang, Jiwei, Zhao, Yongzhao, Chen, Tao, Xiang, Yi, Hui, Jialiang, Cai, Danxian, Liu, Yanna, Xia, Lei, Yu, Tao, Li, Guoxin
Format: Article
Language:English
Subjects:
Animals
Clustered Regularly Interspaced Short Palindromic Repeats
Genetic Engineering
Genomics
Humans
Mutagenesis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:CRISPR technology has rapidly changed the face of biological research, such that precise genome editing has now become routine for many labs within several years of its initial development. CRISPR/Cas9 (Clustered Regularly Interspace Short Palindromic Repeat/CRISPR-associated nuclease 9) gene editing system is a powerful and groundbreaking programmable genome editing technology developed based on an adaptive bacterial and archaea immune system resisting the invasion of exogenous nucleic acid. Compared with traditional genome editing technology, CRISPR/Cas9 system is easier, efficient and less cytotoxic. CRISPR/Cas9 gene editing technology has been applied to many aspects of cancer research, including research on tumor genes, constructing animal tumor models, screening tumor phenotypic-related and resistance-associated gene and cancer gene therapy. In this review, we focus on the application of the CRISPR screen for this fast moving field. Finally, we discuss practical aspects of screen design, and outline a further step forward in the rapidly expanding field of genome editing.
ISSN:2041-2649
2041-2657
DOI:10.1093/bfgp/elw020