Molecular Signals Involved in Human B Cell Migration into the Retina: In Vitro Investigation of ICAM-1, VCAM-1, and CXCL13

Purpose: B cells participate in diverse retinal immunopathologies. Endothelial adhesion molecules and chemokines direct leukocyte trafficking. We examined the involvement of three molecular signals in retinal transendothelial migration of human B cells: ICAM-1, VCAM-1, and CXCL13. Methods: Periphera...

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Bibliographic Details
Published in:Ocular immunology and inflammation 2017-11, Vol.25 (6), p.811-819
Main Authors: Bharadwaj, Arpita S., Stempel, Andrew J., Olivas, Antoinette, Franzese, Samone E., Ashander, Liam M., Ma, Yuefang, Lie, Shervi, Appukuttan, Binoy, Smith, Justine R.
Format: Article
Language:English
Subjects:
B cell
B-Lymphocytes - physiology
Cell Movement - physiology
Chemokine CXCL13 - physiology
CXCL
Endothelium, Vascular - metabolism
human
Humans
ICAM-1
Immunohistochemistry
Immunophenotyping
Intercellular Adhesion Molecule-1 - physiology
Retina - physiology
retina13
Retinal Pigment Epithelium - physiology
Retinal Vessels
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction
Transendothelial and Transepithelial Migration - physiology
Vascular Cell Adhesion Molecule-1 - physiology
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Summary:Purpose: B cells participate in diverse retinal immunopathologies. Endothelial adhesion molecules and chemokines direct leukocyte trafficking. We examined the involvement of three molecular signals in retinal transendothelial migration of human B cells: ICAM-1, VCAM-1, and CXCL13. Methods: Peripheral blood B cells were isolated by negative selection. Migration was studied in transwells populated with human retinal endothelial monolayers, using antibody to block ICAM-1 or VCAM-1. Retinal expression of CXCL13 was investigated. Results: B cells crossed retinal endothelium. ICAM-1 blockade significantly reduced migration when results for all subjects were combined, and for a majority when results were analyzed by individual. This effect was irrespective of the presence or absence of CXCL13, although CXCL13 increased migration. CXCL13 was detected in neural retina and retinal pigment epithelium. Endothelial cells of some retinal vessels presented CXCL13 protein. Conclusion: ICAM-1 blockade may be an effective treatment in some patients with retinal diseases that involve B cells.
ISSN:0927-3948
1744-5078
DOI:10.1080/09273948.2016.1180401