Novel perfusion-decellularized method to prepare decellularized ureters for ureteral tissue-engineered repair

As the endoscopic technique is widely used in the diagnosis and treatment of diseases, the incidence of ureteral injuries increases annually. The classical surgical therapies are not always satisfactory. With the constant development of the tissue engineering technology in the field of urinary recon...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2016-12, Vol.122 (6), p.758-764
Main Authors: Xiao, Shu-wei, Wang, Peng-chao, Fu, Wei-jun, Wang, Zhong-xin, Li, Gang, Zhang, Xu
Format: Article
Language:English
Subjects:
Animals
Cell Survival
Cells, Cultured
Collagen - analysis
Decellularized
Dogs
Extracellular Matrix - chemistry
Feasibility Studies
Glycosaminoglycans - analysis
Guided Tissue Regeneration - instrumentation
Guided Tissue Regeneration - methods
Microscopy, Electron, Scanning
Perfusion
Perfusion system
Repair
Tissue engineering
Tissue Engineering - methods
Tissue Scaffolds - chemistry
Ureter
Ureter - cytology
Ureter - physiology
Ureter - transplantation
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Summary:As the endoscopic technique is widely used in the diagnosis and treatment of diseases, the incidence of ureteral injuries increases annually. The classical surgical therapies are not always satisfactory. With the constant development of the tissue engineering technology in the field of urinary reconstruction, the ureteral reconstruction has become possible technology. In this study, a novel perfusion-decellularized protocol, which combined a perfusion system with the commonly used physical and chemical methods, was used to prepare the decellularized ureters for ureteral reconstruction and the urinary tract-derived cells (UDCs) were seeded on the surface of the perfusion-decellularized ureters (PDUs) in order to observe the cells survival, adhesion, proliferation and distribution. The data of H&E staining, DAPI staining, and the agarose gel electrophoresis demonstrated that the cellular components of PDUs were removed, and the decellularized time was shorter than previous study. In addition, compared with the native ureters, the DNA content of the PDUs was significantly decreased just two percent residue (P
ISSN:1389-1723
1347-4421
DOI:10.1016/j.jbiosc.2016.06.009