Hibiscus sabdariffa increases hydroxocobalamin oral bioavailability and clinical efficacy in vitamin B12 deficiency with neurological symptoms

The aim of the study was to evaluate the bioavailability and clinical benefits of oral new formulation (HB12) of hydroxocobalamin (Hdrx) with Hibiscus sabdariffa (HS). First, in an observational study, a cohort of 30 vitamin B12‐deficient patients (vit B12 < 200 pg/mL) with neurological symptoms...

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Published in:Fundamental & clinical pharmacology 2016-12, Vol.30 (6), p.568-576
Main Authors: Souirti, Zouhayr, Loukili, Mouna, Soudy, Imar D., Rtibi, Kaies, Özel, Aslihan, Limas-Nzouzi, Nicolas, El Ouezzani, Seloua, Eto, Bruno
Format: Article
Language:English
Subjects:
cobalamin
Hibiscus sabdariffa
hydroxocobalamin
intestinal bioavailability
ionic conductance
mice
neurological deficit
pernicious anaemia
pharmacological enhancer
vitamin B12
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Summary:The aim of the study was to evaluate the bioavailability and clinical benefits of oral new formulation (HB12) of hydroxocobalamin (Hdrx) with Hibiscus sabdariffa (HS). First, in an observational study, a cohort of 30 vitamin B12‐deficient patients (vit B12 < 200 pg/mL) with neurological symptoms received oral fixed dose of Hdrx containing 15 mg Hdrx daily for 10 days followed by 15 mg monthly. Clinical benefits were evaluated on haematological and biochemical parameters, and neurological improvement at days 10 and 90 compared to day 0. To understand the mechanism, intestinal mucosa from mice were mounted in vitro in Ussing chambers to measure Hdrx Fluxes. In the clinical study, serum vitamin B12 level increased from 55.1 ± 36.9 to 1330 ± 335.5 pg/mL at day 10 and 431.0 ± 24.27 pg/mL at day 90, without overt adverse effects. In mice ileum, (i) intestinal bioavailability of Hdrx increased in dose‐dependent manner with HB12. The apparent permeability of Hdrx was Papp = 34.9 ± 4.6 × 10−6 cm/s in the presence of 3 mg/mL (HB12B) compared to the control Papp = 6.2 ± 0.7 × 10−6 cm/s. (ii) Total transepithelial electrical conductance (Gt) increased in dose‐dependent manner with HB12, Gt = 161.5 ± 10.8 mS/cm² with HB12B (Hdrx 1 mg + HS 3 mg) compared to the control Hdrx, Gt = 28.7 ± 4.0 mS/cm². In conclusion, the clinical study suggests that injections are not required when Hdrx is given orally. Intestinal bioavailability of Hdrx increased in vitro when it was used concomitantly with HS.
ISSN:0767-3981
1472-8206
DOI:10.1111/fcp.12220