Novel fabrication method of the peritoneal dialysis filter using silk fibroin with urease fixation system

• Published in: Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2017-10, Vol.105 (7), p.2136-2144
• Main Authors: Moon, Bo Mi, Choi, Myung-Jin, Sultan, Md Tipu, Yang, Jae Won, Ju, Hyung Woo, Lee, Jung Min, Park, Hyun Jung, Park, Ye Ri, Kim, Soo Hyeon, Kim, Dong Wook, Lee, Min Chae, Jeong, Ju Yeon, Lee, Ok Joo, Sung, Gun Yong, Park, Chan Hum
• Format: Article
• Language: English
• Subjects: Animals; Biocompatibility; Biomedical materials; Cytotoxicity; Dialysis; End-stage renal disease; Enzymatic activity; Enzyme activity; Enzymes; Enzymes, Immobilized - chemistry; Fabrication; Fibroins - chemistry; Filtration; Fixation; Kidney diseases; Kidney transplantation; Materials research; Materials science; Materials Testing; Mice; NIH 3T3 Cells; Optimization; Peritoneal dialysis; Peritoneal Dialysis - instrumentation; Peritoneum; Porosity; Regeneration; Scaffolds; Silk; Silk fibroin; Toxicity; Urea; Urease; Urease - chemistry; Wearable technology
• ISSN: 1552-4973; 1552-4981
• DOI: 10.1002/jbm.b.33751

During the last decade, there has been a great advance in the kidney dialysis system by wearable artificial kidney (WAK) system for end-stage renal disease patients. Uremic solute removal and water regeneration system are the most prerequisite for WAK to work properly. In this study, we designed a filtering membrane system by using immobilized urease silk fibroin filter and evaluated its comparative effectiveness with a PVDF filtering system in peritoneal dialysate regeneration system by urea removal efficacy. We evaluated this membrane's characteristic and performances by conducting SEM-EDX analyze, water-binding abilities and porosity test, removal abilities of urea, cytotoxicity assay and enzyme activity assay. Under the condition for optimization of urease, the percentage removal of urea was about 40% and 60% in 50 mg/dL urea solution by urease immobilized PVDF and silk fibroin scaffolds, respectively. The batch experimental result showed that immobilized filter removed more than 50% of urea in 50 mg/dL urea solution. In addition silk fibroin with urease filter removed 90 percent of urea in the peritoneal dialysate after 24 h filtration. We suggest that silk fibroin with urease fixation filter can be used more effectively for peritoneal dialysate regeneration system, which have hydrophilic property and prolonged enzyme activity. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2136-2144, 2017.