Low-Volume Label-Free Detection of Molecule-Protein Interactions on Microarrays by Imaging Reflectometric Interferometry

This system allows the high-throughput protein interaction analysis on microarrays. We apply the interference technology 1λ–imaging reflectometric interferometry (iRIf) as a label-free detection method and create microfluidic flow cells in microscope slide format for low reagent consumption and lab...

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Bibliographic Details
Published in:SLAS technology 2017-08, Vol.22 (4), p.437-446
Main Authors: Burger, Juergen, Rath, Christin, Woehrle, Johannes, Meyer, Philipp A., Ben Ammar, Nessim, Kilb, Normann, Brandstetter, Thomas, Pröll, Florian, Proll, Guenther, Urban, Gerald, Roth, Guenter
Format: Article
Language:English
Subjects:
high-throughput protein analysis
imaging label-free detection
microfluidic biosensor
reflectometric interferometry
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Summary:This system allows the high-throughput protein interaction analysis on microarrays. We apply the interference technology 1λ–imaging reflectometric interferometry (iRIf) as a label-free detection method and create microfluidic flow cells in microscope slide format for low reagent consumption and lab work compatibility. By now, most prominent for imaging label-free interaction analyses on microarrays are imaging surface plasmon resonance (SPR) methods, quartz crystal microbalance, or biolayer interferometry. SPR is sensitive against temperature drifts and suffers from plasmon crosstalk, and all systems lack array size (maximum 96 spots). Our detection system is robust against temperature drifts. Microarrays are analyzed with a spatial resolution of 7 µm and time resolution of ≤50 fps. System sensitivity is competitive, with random noise of
ISSN:2472-6303
2472-6311
DOI:10.1177/2211068216657512