Determination of 21 drugs in oral fluid using fully automated supported liquid extraction and UHPLC‐MS/MS

Collection of oral fluid (OF) is easy and non‐invasive compared to the collection of urine and blood, and interest in OF for drug screening and diagnostic purposes is increasing. A high‐throughput ultra‐high‐performance liquid chromatography‐tandem mass spectrometry method for determination of 21 dr...

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Bibliographic Details
Published in:Drug testing and analysis 2017-05, Vol.9 (5), p.808-823
Main Authors: Valen, Anja, Leere Øiestad, Åse Marit, Strand, Dag Helge, Skari, Ragnhild, Berg, Thomas
Format: Article
Language:English
Subjects:
13C‐labelled internal standards
Amphetamine - analysis
Analgesics, Opioid - analysis
automated supported liquid extraction
Automation
Benzodiazepines - analysis
Cannabinoids - analysis
Chromatography, High Pressure Liquid - methods
Cocaine - analysis
Drug testing
drugs of abuse
Humans
Ions
Limit of Detection
Liquid-Liquid Extraction - methods
oral fluid
Pharmaceutical Preparations - analysis
Reproducibility of Results
Saliva - chemistry
Street Drugs - analysis
Substance Abuse Detection - methods
Tandem Mass Spectrometry - methods
UHPLC‐MS/MS
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Summary:Collection of oral fluid (OF) is easy and non‐invasive compared to the collection of urine and blood, and interest in OF for drug screening and diagnostic purposes is increasing. A high‐throughput ultra‐high‐performance liquid chromatography‐tandem mass spectrometry method for determination of 21 drugs in OF using fully automated 96‐well plate supported liquid extraction for sample preparation is presented. The method contains a selection of classic drugs of abuse, including amphetamines, cocaine, cannabis, opioids, and benzodiazepines. The method was fully validated for 200 μL OF/buffer mix using an Intercept OF sampling kit; validation included linearity, sensitivity, precision, accuracy, extraction recovery, matrix effects, stability, and carry‐over. Inter‐assay precision (RSD) and accuracy (relative error) were
ISSN:1942-7603
1942-7611
DOI:10.1002/dta.2045