Adipokines induce pro‐inflammatory factors in activated Cd4+ T cells from osteoarthritis patient

ABSTRACT Osteoarthritis (OA) is a chronic systemic musculoskeletal disorder involving inflammation, immunity, and metabolic alterations. OA is commonly regarded as non‐inflammatory disease; still inflammation is recognized as contributing to the symptoms and progression of OA. New evidence suggests...

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Published in:Journal of orthopaedic research 2017-06, Vol.35 (6), p.1299-1303
Main Authors: Scotece, Morena, Pérez, Tamara, Conde, Javier, Abella, Vanessa, López, Veronica, Pino, Jesús, Gonzalez‐Gay, Miguel A., Gomez‐Reino, Juan J., Mera, Antonio, Gomez, Rodolfo, Gualillo, Oreste
Format: Article
Language:English
Subjects:
adipokines
Adiponectin - physiology
Adult
Aged
Aged, 80 and over
Case-Control Studies
CD4-Positive T-Lymphocytes - metabolism
chondrocytes
Chondrocytes - metabolism
Coculture Techniques
Humans
immunity
inflammation
Leptin - physiology
Lipocalin-2 - physiology
lymphocytes
osteoarthritis
Osteoarthritis - immunology
Primary Cell Culture
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Summary:ABSTRACT Osteoarthritis (OA) is a chronic systemic musculoskeletal disorder involving inflammation, immunity, and metabolic alterations. OA is commonly regarded as non‐inflammatory disease; still inflammation is recognized as contributing to the symptoms and progression of OA. New evidence suggests that adipokines are involved in the pathophysiology of OA and might modulate the production of inflammatory mediators including in immune cells. However, the role of immune component in osteoarthritis is still poorly investigated. To gain further insights into the interaction of immune cells in OA and the role of adipokines on these cells, we performed experiments aimed to determine the cytokine profile in activated CD4+ T cells from OA patients. For completeness, we also explored the cross talk between T lymphocytes and chondrocytes in OA by co‐culturing human primary chondrocytes with activated CD4+ T cells in two ways: the first by incubating the cells by direct contact (D.C.) or by transwell system. Our results show that the exposure of activated CD4+ T cells to adipokines modulates IL‐6, IL‐8, and CCL‐3 production. In addition, the production of key macromolecules of ECM (aggrecan and collagen‐2) and matrix metalloproteinase 13 (MMP‐13) in co‐cultured chondrocytes with activated CD4+ T cells was altered. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:1299–1303, 2017.
ISSN:0736-0266
1554-527X
DOI:10.1002/jor.23377