System-level genome editing in microbes

Highlights • Innovative new technologies allow system-level, high throughput genome editing. • Recombineering offers high specificity and multiplexing ability. • RNA-guided nucleases can act alone or boost the efficiency of recombineering. • Diverse genomic libraries are readily obtainable. • High t...

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Bibliographic Details
Published in:Current opinion in microbiology 2016-10, Vol.33, p.113-122
Main Authors: Csörgő, Bálint, Nyerges, Ákos, Pósfai, György, Fehér, Tamás
Format: Article
Language:English
Subjects:
Bacteria - genetics
Clustered Regularly Interspaced Short Palindromic Repeats - genetics
CRISPR-Cas Systems - genetics
Endonucleases - metabolism
Fungi - genetics
Gene Editing - methods
Genetic Engineering - methods
Genome, Bacterial - genetics
Genome, Fungal - genetics
Genomic Library
Pathology
RNA, Guide, CRISPR-Cas Systems
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Summary:Highlights • Innovative new technologies allow system-level, high throughput genome editing. • Recombineering offers high specificity and multiplexing ability. • RNA-guided nucleases can act alone or boost the efficiency of recombineering. • Diverse genomic libraries are readily obtainable. • High throughput analysis of the libraries needs further innovations.
ISSN:1369-5274
1879-0364
DOI:10.1016/j.mib.2016.07.005