Molecular Dissection of Alleles of the osmotic-1 Locus of Neurospora crassa

Two-component histidine kinases of bacteria, plants, and fungi are involved in the regulation of intracellular events in response to changes in external environmental conditions. Fungal histidine kinases play important roles in osmoregulation, in vivo and in planta virulence, and sensitivity to cert...

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Bibliographic Details
Published in:Fungal genetics and biology 2002-03, Vol.35 (2), p.147-155
Main Authors: Miller, Tamara K, Renault, Samantha, Selitrennikoff, Claude P
Format: Article
Language:English
Subjects:
Alleles
Aminoimidazole Carboxamide - analogs & derivatives
Aminoimidazole Carboxamide - pharmacology
Bridged Bicyclo Compounds - pharmacology
coiled-coil
Drug Resistance, Fungal
Fungicides, Industrial - pharmacology
Histidine Kinase
Hydantoins
linker region
Molecular Sequence Data
Mutation
Neurospora crassa
Neurospora crassa - drug effects
Neurospora crassa - genetics
Neurospora crassa - growth & development
OS-1 gene
OS1 gene
Osmotic Pressure
osmotic-1 (OS-1)
Oxazoles - pharmacology
Protein Kinases - genetics
Sodium Chloride - pharmacology
two-component histidine kinases
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Summary:Two-component histidine kinases of bacteria, plants, and fungi are involved in the regulation of intracellular events in response to changes in external environmental conditions. Fungal histidine kinases play important roles in osmoregulation, in vivo and in planta virulence, and sensitivity to certain classes of antifungals. The osmotic-1 (OS-1) locus of Neurospora crassa encodes a predicted protein with homology to histidine kinases and appears to be an osmosensor. Mutants of the OS-1 locus are hypersensitive to salt and are strongly resistant to dicarboximide antifungals. Molecular analysis of each of eight OS-1 mutants revealed that seven resulted from amino acid changes in a domain of the protein known as the linker region. These results indicate that the linker region of fungal two-component histidine kinases is essential for proper functioning of the kinase.
ISSN:1087-1845
1096-0937
DOI:10.1006/fgbi.2001.1306