Inhibitory effects of rHP-NAP IgY against Helicobacter pylori attachment to AGS cell line

• Published in: Microbial pathogenesis 2016-08, Vol.97, p.231-235
• Main Authors: Borhani, Katayoun, Mohabati Mobarez, Ashraf, Khabiri, Ali Reza, Behmanesh, Mehrdad, Khoramabadi, Nima
• Format: Article
• Language: English
• Subjects: Alternative therapy; Animals; Bacterial Adhesion - drug effects; Cell Line, Tumor; Chickens; Epithelial Cells - microbiology; Gastrointestinal disease; Helicobacter pylori; Helicobacter pylori - drug effects; Helicobacter pylori - immunology; HP-NAP; Humans; IgY; Immunoglobulins - isolation & purification; Immunoglobulins - metabolism; Immunologic Factors - isolation & purification; Immunologic Factors - metabolism; Immunotherapy; Spiperone - analogs & derivatives; Virulence Factors - antagonists & inhibitors; Virulence Factors - immunology
• ISSN: 0882-4010; 1096-1208
• DOI: 10.1016/j.micpath.2016.06.004

Helicobacter pylori is a major human pathogen related to gastric adenocarcinoma and gastroduodenal diseases. Treatment of H. pylori infections is complicated by the rise of antibiotic resistance, necessitating investigation of alternative therapies. One such alternative is passive immunization by oral administration of antibacterial immunoglobulin. In the present study, chicken immunoglobulin (IgY) was used for passive immunotherapy against a major virulence factor of H. pylori, namely recombinant HP-Nap protein. Recombinant HP-Nap was prepared and used to immunize hens. IgY was purified from the eggs by polyethylene glycol precipitation method with a total IgY-HP-NAP yield of 30 mg per egg. The inhibitory effect of specific IgY on H. pylori attachment was investigated in AGS cell line infected by the bacteria. The results demonstrate the potent effect of IgY- HP-NAP in inhibition of H. pylori attachment to the AGS cells. •Treatment of Helicobacter pylori infections is complicated by the rise of antibiotic resistance.•Passively administered IgY has been reported to provide effective protection against enteric pathogens.•Chicken immunoglobulin (IgY) was used for passive immunotherapy.•HP-NAP is one of the main virulence factors in H. pylori that can mediate the binding of bacteria to the cell surface via interaction with carbohydrates of epithelial cells.•Anti-chicken HP-NAP has the potential to be incorporated in large scale production of associated therapeutics for H. pylori due to the high yield and cost effective nature.