Diagnosis, gB genotype distribution and viral load of symptomatic congenitally infected CMV patients in Cuba

Objective: Cytomegalovirus (CMV) is the leading cause of viral congenital infection. Some viral factors have been proposed to be CMV pathogenicity markers. The objective of this study was to investigate the frequency of congenital CMV infection in symptomatic patients and the possible association wi...

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Bibliographic Details
Published in:Journal of perinatology 2016-10, Vol.36 (10), p.837-842
Main Authors: Correa, C, Kourí, V, Pérez, L, Soto, Y, Limia, C
Format: Article
Language:English
Subjects:
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Amniotic fluid
Biomarkers - analysis
Confidence intervals
Congenital diseases
Congenital infection
Consent
Cuba - epidemiology
Cytomegalovirus
Cytomegalovirus - genetics
Cytomegalovirus infections
Cytomegalovirus Infections - congenital
Cytomegalovirus Infections - diagnosis
Cytomegalovirus Infections - epidemiology
Cytomegalovirus Infections - virology
DNA, Viral - analysis
Female
Fetuses
Genetic aspects
Genotype
Genotype & phenotype
Genotypes
Genotyping
Glycoprotein B
Glycoproteins
Herpesviridae
Humans
Infant, Newborn
Infection
Infections
Jaundice
Load distribution
Medical diagnosis
Medical research
Medicine
Medicine & Public Health
Movie theaters
Multiplex Polymerase Chain Reaction - methods
Neonates
original-article
Pathogenicity
Pathogens
Patients
Pediatric Surgery
Pediatrics
Polymerase chain reaction
Pregnancy
Pregnant women
Saliva
Sepsis
Sexually transmitted diseases
Splenomegaly
Statistical analysis
Statistical methods
Statistics, Nonparametric
STD
Ultrasonography, Prenatal
Urine
Vagina
Viral Envelope Proteins - genetics
Viral Load
Virology
Womens health
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Summary:Objective: Cytomegalovirus (CMV) is the leading cause of viral congenital infection. Some viral factors have been proposed to be CMV pathogenicity markers. The objective of this study was to investigate the frequency of congenital CMV infection in symptomatic patients and the possible association with the CMV glycoprotein B (gB) genotype and viral load. Study Design: A total of 361 newborns (NB) and 158 pregnant women (PW) with clinically suspected CMV infection were enrolled. Studied samples included urine, saliva, serum, vaginal swabs and amniotic fluid. CMV infection was diagnosed by multiplex nested PCR. CMV gB genotyping was performed on infected samples, followed by viral load determination. Results: Overall, 18.7% of the tested patients were positive for CMV infection, 19.7% of NB were congenitally infected and 16.5% of PW showed active CMV infection. gB-2 was the most prevalent genotype detected (39/97 patients). gB CMV mixed infections were detected in 12 patients. gB-2 was associated with mono-infections ( P
ISSN:0743-8346
1476-5543
DOI:10.1038/jp.2016.95