Utilizing metal tolerance potential of soil fungus for efficient synthesis of gold nanoparticles with superior catalytic activity for degradation of rhodamine B

• Published in: Journal of environmental management 2016-12, Vol.183, p.22-32
• Main Authors: Bhargava, Arpit, Jain, Navin, Khan, Mohd Azeem, Pareek, Vikram, Dilip, R. Venkataramana, Panwar, Jitendra
• Format: Article
• Language: English
• Subjects: Biocatalysts; Biodegradation; Biosynthesis; Borohydrides - chemistry; Catalysis; Cladosporium; Cladosporium - metabolism; Fungi; Fungus; Gold - chemistry; Gold - metabolism; Gold nanoparticles; Metal Nanoparticles - chemistry; Nanomaterials; Optimization; Organic dye; Oxidation-Reduction; Particle Size; Physical chemistry; Remediation; Rhodamines - chemistry; Soil Microbiology; Sustainable development
• ISSN: 0301-4797; 1095-8630
• DOI: 10.1016/j.jenvman.2016.08.021

In recent years, the surging demand of nanomaterials has boosted unprecedented expansion of research for the development of high yielding and sustainable synthesis methods which can deliver nanomaterials with desired characteristics. Unlike the well-established physico-chemical methods which have various limitations, biological methods inspired by mimicking natural biomineralization processes have great potential for nanoparticle synthesis. An eco-friendly and sustainable biological method that deliver particles with well-defined shape, size and compositions can be developed by selecting a proficient organism followed by fine tuning of various process parameter. The present study revealed high metal tolerance ability of a soil fungus Cladosporium oxysporum AJP03 and its potential for extracellular synthesis of gold nanoparticles. The morphology, composition and crystallinity of nanoparticles were confirmed using standard techniques. The synthesized particles were quasi-spherical in shape with fcc packing and an average particle size of 72.32 ± 21.80 nm. A series of experiments were conducted to study the effect of different process parameters on particle size and yield. Biomass: water ratio of 1:5 and 1 mM precursor salt concentration at physiological pH (7.0) favoured the synthesis of well-defined gold nanoparticles with maximum yield. The as-synthesized nanoparticles showed excellent catalytic efficiency towards sodium borohydride mediated reduction of rhodamine B (2.5 × 10−5 M) within 7 min of reaction time under experimental conditions. Presence of proteins as capping material on the nanoparticle surface was found to be responsible for this remarkable catalytic efficiency. The present approach can be extrapolated to develop controlled and up-scalable process for mycosynthesis of nanoparticles for diverse applications. [Display omitted] •The extracellular biosynthesis of gold nanoparticles was performed.•Metal tolerant soil fungus Cladosporium oxysporum AJP03 was utilized for synthesis.•Effect of various process parameters on nanoparticle yield and size were studied.•Gold nanoparticles catalyzed reduction of rhodamine B was performed.•Proteins on nanoparticle surface were responsible for superior catalytic activity.