Abstract # 1810 Exercise induces a differential mobilisation of haematopoietic stem cell subsets into peripheral blood which is partly influenced by donor age

• Published in: Brain, behavior, and immunity behavior, and immunity, 2016-10, Vol.57, p.e29-e29
• Main Authors: Brown, F.F, Bosch, J.A, Aldred, S, Drayson, M.T, Turner, J.E
• Format: Article
• Language: English
• Subjects: Allergy and Immunology; Psychiatry
• ISSN: 0889-1591; 1090-2139
• DOI: 10.1016/j.bbi.2016.07.098

Exercise-induced leukocyte mobilisation is well defined, but few studies have examined their precursors – haematopoietic stem cells (HSCs). Seventeen males (33 ± 13 years, BMI 24 ± 3 kg·m2 and VO2max 60 ± 5 ml·kg·min) ran for 60 min at 80% VO2max. Blood was collected at rest, during the final minute of exercise, then 15, 60 and 120 min later. HSC populations were identified using flow cytometry: HSCs (CD34+CD45dim ); Common Lymphoid Progenitor cells (CLP, CD34+CD45dim CD38-CD7+); Common Myeloid Progenitor cells (CMP, CD34+CD45dim CD38+CD123+CD45RA-); Megakaryocyte/Erythroid Progenitor cells (MEP, CD34+CD45dim CD38+CD123-CD45RA-); and Granulocyte/Macrophage Progenitor cells (GMP, CD34+CD45dim CD38+CD123+CD45RA+). HSCs increased by 100% during exercise, returning to baseline within 15 min, and fell 10% below baseline 60–120 min post-exercise ( p < 0.05, η 2 = 0.44). CLP, CMP and GMPs were un-changed during or following exercise, but CMPs and GMPs fell 30–40% below baseline 1–2 h later (p’s 0.05; η 2 = 0.14), suggesting the smaller HSC mobilisation in older men was partly due to lower cardiac output and concomitant haemodynamic/shear forces. Exercise-induced HSC mobilisation could increase apheresis yields for transplant, but compared to younger donors, the elderly may require a more prolonged stimulus.