Simultaneous estimation of levodopa, carbidopa and 3-oxymethyldopa in rat plasma using HPLC-ECD

The aim of study was to develop a suitable analytical method for simultaneous estimation of levodopa, carbidopa and 3‐O‐methyl dopa in rat plasma. Chromatographic separation of plasma samples was achieved using a reverse‐phase C18 column. The mobile phase used consisted of a mixture of methanol and...

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Bibliographic Details
Published in:Biomedical chromatography 2016-10, Vol.30 (10), p.1696-1700
Main Authors: Raut, Prashant P., Charde, Shrikant Y., Bishnoi, Pradeep
Format: Article
Language:English
Subjects:
3-O-methyl dopa
Animals
bioanalytical method
carbidopa
Carbidopa - blood
Chromatography, High Pressure Liquid - methods
Dihydroxyphenylalanine - analogs & derivatives
Dihydroxyphenylalanine - blood
Electrochemical Techniques - methods
HPLC-ECD
levodopa
Levodopa - blood
Limit of Detection
Male
Rats
Rats, Wistar
Tyrosine - analogs & derivatives
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Summary:The aim of study was to develop a suitable analytical method for simultaneous estimation of levodopa, carbidopa and 3‐O‐methyl dopa in rat plasma. Chromatographic separation of plasma samples was achieved using a reverse‐phase C18 column. The mobile phase used consisted of a mixture of methanol and phosphate buffer (10 mM, pH 3.50) in the ratio of 90:10 v/v. All analytes were estimated by electrochemical detection at +800 mV. The developed method has been validated as per the standard guidelines. Precision study results were found to be satisfactory, with percentage relative standard deviation for repeatability and intermediate precision
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.3713