Elevated levels of macromolecular damage are correlated with increased nitric oxide synthase expression in erythrocytes isolated from twin neonates

Summary Pregnancy is a state associated with an enhanced metabolism and demand for O2, which may lead to the overproduction of reactive oxygen species (ROS) and hence to oxidative stress. An elevated ROS level may result in delayed development and a low birth weight. The aim of this study was to rev...

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Bibliographic Details
Published in:British journal of haematology 2016-09, Vol.174 (6), p.932-941
Main Authors: Dugmonits, Krisztina N., Ferencz, Ágnes, Zahorán, Szabolcs, Lázár, Renáta, Talapka, Petra, Orvos, Hajnalka, Hermesz, Edit
Format: Article
Language:English
Subjects:
Adult
Antioxidants - metabolism
Birth Weight
Cell Membrane - metabolism
endothelial nitric oxide synthase
Enzyme Activation
erythrocyte
Erythrocytes - metabolism
Female
Gene Expression
Gestational Age
Glutathione Disulfide
Humans
Infant, Newborn
Lipid Peroxidation
Nitric Oxide - metabolism
Nitric Oxide Synthase - genetics
Nitric Oxide Synthase - metabolism
Nitric Oxide Synthase Type III
Oxidation-Reduction
Oxidative Stress
Pregnancy
protein nitration
Reactive Oxygen Species - metabolism
Twins
umbilical cord blood
Young Adult
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Summary:Summary Pregnancy is a state associated with an enhanced metabolism and demand for O2, which may lead to the overproduction of reactive oxygen species (ROS) and hence to oxidative stress. An elevated ROS level may result in delayed development and a low birth weight. The aim of this study was to reveal the consequences of multiple pregnancies on the redox status of neonatal human red blood cells (RBCs) and evaluate the role of endothelial nitric oxide synthase (NOS3) – expressing RBCs in the generation of oxidative stress. The study presents evidence of higher levels of production of hydrogen peroxide, peroxynitrite and nitrate content in the RBCs of twin neonates, clearly reflected by an elevated level of protein and lipid damages. This phenotype appears to be a consequence of multiple pregnancies, regardless of the level of maturity or the birth weight of the twins. Besides the higher level of ROS, there was a general decrease in the expression of genes coding for antioxidants. The first data are presented on NOS3‐expressing neonatal human RBCs. The number of RBCs producing NOS3 was more than twice as high in twin neonates compared to singletons, with no correlation to maturity.
ISSN:0007-1048
1365-2141
DOI:10.1111/bjh.14156