Genetic variability of the stable fly assessed on a global scale using amplified fragment length polymorphism

• Published in: Insect science 2016-10, Vol.23 (5), p.695-703
• Main Authors: Kneeland, Kathleen M., Skoda, Steven R., Foster, John E.
• Format: Article
• Language: English
• Subjects: AFLP; Amplified Fragment Length Polymorphism Analysis; Animals; biting flies; Diptera; external parasites; Gene Flow; Genetic diversity; Genetic Drift; Genetic Variation; Genotype & phenotype; livestock pests; Muscidae; Muscidae - genetics; Stomoxys calcitrans
• ISSN: 1744-7917; 1672-9609; 1744-7917
• DOI: 10.1111/1744-7917.12219

The stable fly, Stomoxys calcitrans (L.) (Diptera: Muscidae), is a blood-feeding, economically important pest of animals and humans worldwide. Improved management strategies are essential and their development would benefit from studies on genetic diversity of stable flies. Especially if done on a global scale, such research could generate information necessary for the development and application of more efficient control methods. Herein we report on a genetic study of stable flies using amplified fragment length polymorphism, with samples of 10–40 individuals acquired from a total of 25 locations in the Nearctic, Neotropic, Palearctic, Afrotropic and Australasian biogeographical regions. We hypothesized that genetic differentiation would exist across geographical barriers. Although FST (0.33) was moderately high, the GST (0.05; representing genetic diversity between individuals) was very low; Nm values (representing gene flow) were high (9.36). The mismatch distribution and tests of neutrality suggested population expansion, with no genetic differentiation between locations. The analysis of molecular variance (AMOVA) results showed the majority of genetic diversity was within groups. The mantel test showed no correlation between geographic and genetic distance; this strongly supports theAMOVA results. These results suggest that stable flies did not show genetic differentiation but are panmictic, with no evidence of isolation by distance or across geographical barriers.