Protein biomarkers and microbial profiles in peri-implantitis

Objectives The aim of the present investigation was to determine the profile of peri‐implant crevicular fluid (PICF) biomarkers combined with microbial profiles from implants with healthy peri‐implant tissues and peri‐implantitis to assess real‐time disease activity. Material and methods Sixty‐eight...

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Bibliographic Details
Published in:Clinical oral implants research 2016-09, Vol.27 (9), p.1129-1136
Main Authors: Wang, Hom-Lay, Garaicoa-Pazmino, Carlos, Collins, Amy, Ong, Hwen-Sei, Chudri, Rini, Giannobile, William V.
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Bacteroides forsythus
Biomarkers - analysis
Cross-Sectional Studies
dental implant microbiology
Dentistry
Female
Gingival Crevicular Fluid - chemistry
Gingival Recession - microbiology
Humans
Interleukin-1beta - analysis
Male
Matrix Metalloproteinase 8 - analysis
Middle Aged
Osteoprotegerin - analysis
peri-implant crevicular fluid
peri-implant disease
Peri-Implantitis - metabolism
Peri-Implantitis - microbiology
Porphyromonas gingivalis
Prevotella intermedia
salivary diagnostics
Tissue Inhibitor of Metalloproteinase-2 - analysis
Treponema denticola
Vascular Endothelial Growth Factor A - analysis
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Summary:Objectives The aim of the present investigation was to determine the profile of peri‐implant crevicular fluid (PICF) biomarkers combined with microbial profiles from implants with healthy peri‐implant tissues and peri‐implantitis to assess real‐time disease activity. Material and methods Sixty‐eight patients were included in this cross‐sectional study. They were divided into two groups: 34 patients with at least one healthy implant (control) and 34 with at least one peri‐implantitis affected implant (test). Total DNA content and qPCR analysis for periodontal bacteria obtained from subgingival plaque samples (Aggregatibacter actinomycetemcomitans, Prevotella intermedia, Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola) and a PICF analysis for IL‐1β, VEGF, MMP‐8, TIMP‐2, and OPG were performed. The individual and combined diagnostic ability of each biomarker for peri‐implantitis and target bacterial species were analyzed. Results The mean concentration of IL‐1β (44.6 vs. 135.8 pg/ml; P 
ISSN:0905-7161
1600-0501
DOI:10.1111/clr.12708