Effect of cadmium on phenolic compounds, antioxidant enzyme activity and oxidative stress in blueberry (Vaccinium corymbosum L.) plantlets grown in vitro

• Published in: Ecotoxicology and environmental safety 2016-11, Vol.133, p.316-326
• Main Authors: Manquián-Cerda, K., Escudey, M., Zúñiga, G., Arancibia-Miranda, N., Molina, M., Cruces, E.
• Format: Article
• Language: English
• Subjects: Antioxidants - pharmacology; Biphenyl Compounds; Blueberry Plants - drug effects; Cadmium; Cadmium - toxicity; Chlorogenic acid; Drug Administration Schedule; Hydrogen Peroxide - pharmacology; LC-MS; Malondialdehyde - metabolism; MDA; Oxidation-Reduction; Oxidative stress; Oxidative Stress - drug effects; Phenolic compounds; Phenols - metabolism; Picrates; Soil Pollutants - chemistry; Soil Pollutants - toxicity; Vaccinium corymbosum
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2016.07.029

Cadmium (Cd2+) can affect plant growth due to its mobility and toxicity. We evaluated the effects of Cd2+ on the production of phenolic compounds and antioxidant response of Vaccinium corymbosum L. Plantlets were exposed to Cd2+ at 50 and 100µM for 7, 14 and 21 days. Accumulation of malondialdehyde (MDA), hydrogen peroxide (H2O2) and the antioxidant enzyme SOD was determined. The profile of phenolic compounds was evaluated using LC-MS. The antioxidant activity was measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and the ferric reducing antioxidant power test (FRAP). Cd2+ increased the content of MDA, with the highest increase at 14 days. The presence of Cd2+ resulted in changes in phenolic compounds. The main phenolic compound found in blueberry plantlets was chlorogenic acid, whose abundance increased with the addition of Cd2+ to the medium. The changes in the composition of phenolic compounds showed a positive correlation with the antioxidant activity measured using FRAP. Our results suggest that blueberry plantlets produced phenolic compounds with reducing capacity as a selective mechanism triggered by the highest activity of Cd2+. •Blueberry plantlets cultivated in vitro, were exposed to 50 and 100µM of Cd.•16 phenolic compounds were identified with the most abundance of Chlorogenic acid.•Blueberry plantlets produced phenolic compounds with reducing capacity at high Cd2+ doses.