Identification of a shootin1 isoform expressed in peripheral tissues

Shootin1 is a brain-specific cytoplasmic protein involved in neuronal polarity formation and axon outgrowth. It accumulates at the leading edge of axonal growth cones, where it mediates the mechanical coupling between F-actin retrograde flow and cell adhesions as a clutch molecule, thereby producing...

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Bibliographic Details
Published in:Cell and tissue research 2016-10, Vol.366 (1), p.75-87
Main Authors: Higashiguchi, Yasuna, Katsuta, Kazuhiro, Minegishi, Takunori, Yonemura, Shigenobu, Urasaki, Akihiro, Inagaki, Naoyuki
Format: Article
Language:English
Subjects:
Actin
Aging - metabolism
Amino Acid Sequence
Animals
Antibodies
Antibody Specificity
Biomedical and Life Sciences
Biomedicine
Cadherins - metabolism
Cell Communication
Cortactin - metabolism
Cytoplasm
Cytoskeleton
Dendritic cells
Human Genetics
Immunohistochemistry
Mice, Inbred C57BL
Molecular Medicine
Muscle proteins
Nerve Tissue Proteins - chemistry
Nerve Tissue Proteins - metabolism
Neurons
Neurophysiology
Organ Specificity
Protein Isoforms
Protein Transport
Proteins
Proteomics
Rats
Regular Article
Skin
Tissue Distribution
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Summary:Shootin1 is a brain-specific cytoplasmic protein involved in neuronal polarity formation and axon outgrowth. It accumulates at the leading edge of axonal growth cones, where it mediates the mechanical coupling between F-actin retrograde flow and cell adhesions as a clutch molecule, thereby producing force for axon outgrowth. In this study, we report a novel splicing isoform of shootin1 which is expressed not only in the brain but also in peripheral tissues. We have renamed the brain-specific shootin1 as shootin1a and termed the novel isoform as shootin1b. Immunoblot and immunohistochemical analyses with a shootin1b-specific antibody revealed that shootin1b is distributed in various mouse tissues including the lung, liver, stomach, intestines, spleen, pancreas, kidney and skin. Interestingly, shootin1b immunoreactivity was widely detected in epithelial cells that constitute simple and stratified epithelia; in some cells, it colocalized with E-cadherin and cortactin at cell–cell contact sites. Shootin1b also localized in dendritic cells in the spleen. These results suggest that shootin1b may function in various peripheral tissues including epithelial cells.
ISSN:0302-766X
1432-0878
DOI:10.1007/s00441-016-2415-9