Hepatitis E virus derived from different sources exhibits different behaviour in virus inactivation and/or removal studies with plasma derivatives

Hepatitis E virus (HEV) causes viral hepatitis, and is considered a risk factor for blood products. Although some HEV inactivation/removal studies have been reported, detailed investigations of different manufacturing steps as heat treatment, partitioning during cold ethanol fractionation, low pH tr...

Full description

Saved in:
Bibliographic Details
Published in:Biologicals 2016-09, Vol.44 (5), p.403-411
Main Authors: Yunoki, Mikihiro, Tanaka, Hiroyuki, Takahashi, Kadue, Urayama, Takeru, Hattori, Shinji, Ideno, Shoji, Furuki, Rie, Sakai, Kaoru, Hagiwara, Katsuro, Ikuta, Kazuyoshi
Format: Article
Language:English
Subjects:
Animals
Disinfection - methods
Dogs
Encephalomyocarditis virus
Ethanol fractionation
Female
Hepatitis E
Hepatitis E virus
Hepatitis E virus - chemistry
Hepatitis E virus - isolation & purification
HEV
Humans
Hydrogen-Ion Concentration
Liquid-heating
Low pH
Male
Mice
Partitioning
Plasma - virology
Plasma derivatives
Swine
Virus Inactivation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Hepatitis E virus (HEV) causes viral hepatitis, and is considered a risk factor for blood products. Although some HEV inactivation/removal studies have been reported, detailed investigations of different manufacturing steps as heat treatment, partitioning during cold ethanol fractionation, low pH treatment, and virus filtration have yet to be reported for plasma-derived medicinal products. In this study, human serum- and swine faeces-derived HEVs, with and without detergent treatment, were used. The kinetic patterns of inactivation, log reduction value, or partitioning during the process were evaluated. In addition, the mouse encephalomyocarditis virus (EMCV) and canine and porcine parvoviruses (CPV/PPV) were also evaluated as model viruses for HEV. Small pore size (19 or 15 nm) virus filtration demonstrated effective removal of HEV. Middle pore size (35 nm) virus filtration and 60 °C liquid heating demonstrated moderate inactivation/removal. Ethanol fractionation steps demonstrated limited removal of HEV. Unpurified HEV exhibited different properties than the detergent-treated HEV, and both forms displayed differences when compared with EMCV, CPV, and PPV. Limited or no inactivation of HEV was observed during low pH treatment. Untreated plasma-derived HEV from humans showed different properties compared to that of HEV treated with detergent or derived from swine faeces. Therefore, HEV spike preparation requires more attention.
ISSN:1045-1056
1095-8320
DOI:10.1016/j.biologicals.2016.05.004